Cancer-associated carbohydrate antigens as potential biomarkers for hepatocellular carcinoma.
Cancer-associated carbohydrate antigens as potential biomarkers for hepatocellular carcinoma.
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DOI：
10.1371/journal.pone.0039466
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yu YL
中科院分区：
文献类型：
--
作者：
Wu CS;Yen CJ;Chou RH;Li ST;Huang WC;Ren CT;Wu CY;Yu YL
肝细胞癌是人类最常见的恶性肿瘤之一。因此，迫切需要开发早期、高灵敏度的诊断标记物来预防肝细胞癌。血清甲胎蛋白是目前使用的临床生物标记物，在∼患者中只有60%升高；因此，其他生物标记物的识别有望对公众健康产生重大影响。在这项研究中，我们使用糖链微阵列分析来探索几种癌症相关碳水化合物抗原(CAAs)作为肝细胞癌生物标志物的潜在诊断价值。我们利用58种不同的糖链类似物的糖链芯片分析方法，对593份人血清样本(293份肝癌样本、133份慢性乙肝病毒感染样本、134份慢性丙型肝炎病毒感染样本和33份健康献血员样本)进行定量比较，以探讨血清抗体变化作为肝细胞癌生物标志物诊断的可能性。肝细胞癌患者血清抗DSGG、抗岩藻糖基GM1和抗GB2抗体水平显著高于非慢性丙型肝炎患者。总体而言，在我们的研究人群中，Cacas的候选生物标记物DSGG、岩藻糖基GM1和GB2获得了比AFP更好的预测敏感性。我们确定了适用于肝细胞癌早期检测的潜在生物标志物。葡聚糖微阵列分析为高敏、高通量检测抗Cacas血清抗体提供了强有力的工具，有望成为肝癌高危人群早期发现的有价值的血清生物标志物。
Hepatocellular carcinoma (HCC) is one of the most common human malignancies. Therefore, developing the early, high-sensitivity diagnostic biomarkers to prevent HCC is urgently needed. Serum a-fetoprotein (AFP), the clinical biomarker in current use, is elevated in only ∼60% of patients with HCC; therefore, identification of additional biomarkers is expected to have a significant impact on public health. In this study, we used glycan microarray analysis to explore the potential diagnostic value of several cancer-associated carbohydrate antigens (CACAs) as biomarkers for HCC. We used glycan microarray analysis with 58 different glycan analogs for quantitative comparison of 593 human serum samples (293 HCC samples; 133 chronic hepatitis B virus (HBV) infection samples, 134 chronic hepatitis C virus (HCV) infection samples, and 33 healthy donor samples) to explore the diagnostic possibility of serum antibody changes as biomarkers for HCC. Serum concentrations of anti-disialosyl galactosyl globoside (DSGG), anti-fucosyl GM1 and anti-Gb2 were significantly higher in patients with HCC than in chronic HBV infection individuals not in chronic HCV infection patients. Overall, in our study population, the biomarker candidates DSGG, fucosyl GM1 and Gb2 of CACAs achieved better predictive sensitivity than AFP. We identified potential biomarkers suitable for early detection of HCC. Glycan microarray analysis provides a powerful tool for high-sensitivity and high-throughput detection of serum antibodies against CACAs, which may be valuable serum biomarkers for the early detection of persons at high risk for HCC.
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影响因子：
--
作者：
de Leoz ML;An HJ;Kronewitter S;Kim J;Beecroft S;Vinall R;Miyamoto S;de Vere White R;Lam KS;Lebrilla C
通讯作者：
Lebrilla C
影响因子：
25.7
作者：
CAPORASO, N;ROMANO, M;COLTORTI, M
通讯作者：
COLTORTI, M
影响因子：
4
作者：
Kobata, A;Amano, J
通讯作者：
Amano, J
影响因子：
4.3
作者：
Chandrasekaran, EV;Chawda, R;Matta, KL
通讯作者：
Matta, KL
影响因子：
5.1
作者：
Marrero, Jorge A
通讯作者：
Marrero, Jorge A