Enhancement of the in vivo osteogenic potential of marrow/hydroxyapatite composites by bovine bone morphogenetic protein.
Enhancement of the in vivo osteogenic potential of marrow/hydroxyapatite composites by bovine bone morphogenetic protein.
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牛骨形态发生蛋白增强骨髓/羟基磷灰石复合材料的体内成骨潜力。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Kunio Yonemasu
中科院分区：
文献类型：
--
作者：
Toshiaki Noshi;T. Yoshikawa;M. Ikeuchi;Y. Dohi;H. Ohgushi;K. Horiuchi;Masahito Sugimura;K. Ichijima;Kunio Yonemasu
骨髓间充质干细胞和多孔羟基磷灰石（HA）的复合物具有体内成骨潜力。为了研究增强骨髓/HA复合材料成骨潜力的因素，我们通过肝素-琼脂糖亲和层析从牛骨的4M胍提取物中制备了骨形态发生蛋白（BMP）组分。将骨髓/HA复合材料或含有骨髓间充质干细胞、BMP和HA的复合材料（骨髓/BMP/HA复合材料）皮下植入7周龄雄性Fischer大鼠体内。还植入了 BMP/HA 复合材料和单独的 HA。 2、4或8周后收获植入物并为组织学和生化研究做好准备。组织学检查显示2周时明显的从头骨形成以及活性成骨细胞，以及4周和8周时在骨髓/BMP/HA复合材料的许多孔中更广泛的骨形成。骨髓/HA复合材料在2周时没有诱导骨形成，但在4周时有中等程度的骨形成。从蛋白质和基因水平上的碱性磷酸酶和骨钙素表达来看，第 2 周时，只有骨髓/BMP/HA 复合材料产生了强烈的成骨活性。这些结果表明，骨髓间充质干细胞、多孔HA和BMP的组合可协同增强成骨潜力，并可能为其临床应用提供合理的基础，尽管还需要进一步的体内实验。
A composite of marrow mesenchymal stem cells and porous hydroxyapatite (HA) has in vivo osteogenic potential. To investigate factors enhancing the osteogenic potential of marrow/HA composites, we prepared a bone morphogenetic protein (BMP) fraction from the 4M guanidine extract of bovine bone by heparin-sepharose affinity chromatography. Marrow/HA composites or composites containing marrow mesenchymal stem cells, BMP, and HA (marrow/BMP/HA composites) were implanted subcutaneously in 7-week-old male Fischer rats. BMP/HA composites and HA alone were also implanted. The implants were harvested after 2, 4, or 8 weeks and were prepared for histological and biochemical studies. Histological examination showed obvious de novo bone formation together with active osteoblasts at 2 weeks, as well as more extensive bone formation at 4 and 8 weeks in many pores of the marrow/BMP/HA composites. The marrow/HA composites did not induce bone formation at 2 weeks, but there was moderate bone formation at 4 weeks. At 2 weeks, only marrow/BMP/HA composites resulted in intensive osteogenic activity, judging from alkaline phosphatase and osteocalcin expression at both the protein and gene levels. These results indicate that the combination of marrow mesenchymal stem cells, porous HA, and BMP synergistically enhances osteogenic potential, and may provide a rational basis for their clinical application, although further in vivo experiment is needed.
影响因子：
2.7
作者：
RICKARD, DJ;SULLIVAN, TA;KAZHDAN, I
通讯作者：
KAZHDAN, I
DOI：
10.1016/s0021-9258(19)69522-0
发表时间：
1981-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Price;J. W. Lothringer;S. Baukol;A. Reddi
通讯作者：
P. Price;J. W. Lothringer;S. Baukol;A. Reddi
影响因子：
15.9
作者：
PRICE, PA;PARTHEMORE, JG;NISHIMOTO, SK
通讯作者：
NISHIMOTO, SK