Excretory/Secretory Proteome of Females and Males of the Hookworm Ancylostoma ceylanicum.
Excretory/Secretory Proteome of Females and Males of the Hookworm Ancylostoma ceylanicum.
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DOI：
10.3390/pathogens12010095
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发表时间：
2023-01-06
期刊：
影响因子：
--
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中科院分区：
文献类型：
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The dynamic host-parasite mechanisms underlying hookworm infection establishment and maintenance in mammalian hosts remain poorly understood but are primarily mediated by hookworm’s excretory/secretory products (ESPs), which have a wide spectrum of biological functions. We used ultra-high performance mass spectrometry to comprehensively profile and compare female and male ESPs from the zoonotic human hookworm Ancylostoma ceylanicum, which is a natural parasite of dogs, cats, and humans. We improved the genome annotation, decreasing the number of protein-coding genes by 49% while improving completeness from 92 to 96%. Compared to the previous genome annotation, we detected 11% and 10% more spectra in female and male ESPs, respectively, using this improved version, identifying a total of 795 ESPs (70% in both sexes, with the remaining sex-specific). Using functional databases (KEGG, GO and Interpro), common and sex-specific enriched functions were identified. Comparisons with the exclusively human-infective hookworm Necator americanus identified species-specific and conserved ESPs. This is the first study identifying ESPs from female and male A. ceylanicum. The findings provide a deeper understanding of hookworm protein functions that assure long-term host survival and facilitate future engineering of transgenic hookworms and analysis of regulatory elements mediating the high-level expression of ESPs. Furthermore, the findings expand the list of potential vaccine and diagnostic targets and identify biologics that can be explored for anti-inflammatory potential.
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影响因子：
14.9
作者：
Blum M;Chang HY;Chuguransky S;Grego T;Kandasaamy S;Mitchell A;Nuka G;Paysan-Lafosse T;Qureshi M;Raj S;Richardson L;Salazar GA;Williams L;Bork P;Bridge A;Gough J;Haft DH;Letunic I;Marchler-Bauer A;Mi H;Natale DA;Necci M;Orengo CA;Pandurangan AP;Rivoire C;Sigrist CJA;Sillitoe I;Thanki N;Thomas PD;Tosatto SCE;Wu CH;Bateman A;Finn RD
通讯作者：
Finn RD
影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
14.9
作者：
Chen T;Ma J;Liu Y;Chen Z;Xiao N;Lu Y;Fu Y;Yang C;Li M;Wu S;Wang X;Li D;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
7.3
作者：
Eichenberger RM;Ryan S;Jones L;Buitrago G;Polster R;Montes de Oca M;Zuvelek J;Giacomin PR;Dent LA;Engwerda CR;Field MA;Sotillo J;Loukas A
通讯作者：
Loukas A
影响因子：
30.8
作者：
International Helminth Genomes Consortium
通讯作者：
International Helminth Genomes Consortium