Arabidopsis class I formins control membrane-originated actin polymerization at pollen tube tips.
Arabidopsis class I formins control membrane-originated actin polymerization at pollen tube tips.
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拟南芥I类formins控制花粉管尖端膜源性肌动蛋白聚合
DOI：
10.1371/journal.pgen.1007789
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发表时间：
2018-11
期刊：
影响因子：
4.5
通讯作者：
Huang S
中科院分区：
文献类型：
--
作者：
Lan Y;Liu X;Fu Y;Huang S
快速极化花粉管生长需要动态顶端肌动蛋白丝群体。然而，驱动它们组装的细胞机制仍不完全清楚。据推测，福尔明是顶端肌动蛋白成核组装的主要参与者，但直接的遗传和细胞学证据仍有待确定。在这里，我们发现拟南芥formin 3（AtFH3）和formin 5（AtFH5）都参与花粉管中顶端肌动蛋白聚合和肌动蛋白阵列构建的调节，其中AtFH3发挥更主导作用。我们发现两种福明都有质膜（PM）定位信号，但在花粉管中表现出不同的PM定位模式，并且它们功能的丧失会减少顶端肌动蛋白丝的数量。活细胞成像显示，丝状肌动蛋白的减少很可能是由于丝伸长率的减少所致。此外，我们发现，在formin功能丧失突变体花粉管中，尖端定向囊泡运输速率降低，顶端囊泡积累模式改变，这在一定程度上解释了花粉管伸长的减少。因此，我们提供了直接的遗传和细胞学证据，表明福尔明在花粉管尖端的 PM 成核肌动蛋白组装中发挥着重要作用。
A population of dynamic apical actin filaments is required for rapid polarized pollen tube growth. However, the cellular mechanisms driving their assembly remain incompletely understood. It was postulated that formin is a major player in nucleating apical actin assembly, but direct genetic and cytological evidence remains to be firmly established. Here we found that both Arabidopsis formin 3 (AtFH3) and formin 5 (AtFH5) are involved in the regulation of apical actin polymerization and actin array construction in pollen tubes, with AtFH3 playing a more dominant role. We found that both formins have plasma membrane (PM) localization signals but exhibit distinct PM localization patterns in the pollen tube, and loss of their function reduces the amount of apical actin filaments. Live-cell imaging revealed that the reduction in filamentous actin is very likely due to the decrease in filament elongation. Furthermore, we found that the rate of tip-directed vesicle transport is reduced and the pattern of apical vesicle accumulation is altered in formin loss-of-function mutant pollen tubes, which explains to some extent the reduction in pollen tube elongation. Thus, we provide direct genetic and cytological evidence showing that formin is an important player in nucleating actin assembly from the PM at pollen tube tips.
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拟南芥formin 2通过加帽和稳定胞间连丝的肌动蛋白丝来调节细胞间运输
影响因子：
7.7
作者：
Diao M;Ren S;Wang Q;Qian L;Shen J;Liu Y;Huang S
通讯作者：
Huang S
影响因子：
11.6
作者：
Favery, B;Chelysheva, LA;Abad, P
通讯作者：
Abad, P
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.4
作者：
Grunt M;Zárský V;Cvrcková F
通讯作者：
Cvrcková F
DOI：
10.1073/pnas.1008527107
发表时间：
2010-09-14
影响因子：
11.1
作者：
Cheung, Alice Y.;Niroomand, Shahriar;Wu, Hen-Ming
通讯作者：
Wu, Hen-Ming