Diagnostic and prognostic role of urinary collagens in primary human bladder cancer.
Diagnostic and prognostic role of urinary collagens in primary human bladder cancer.
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DOI：
10.1111/cas.13384
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发表时间：
2017-11
期刊：
影响因子：
5.7
通讯作者：
Fujimoto K
中科院分区：
文献类型：
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作者：
Miyake M;Morizawa Y;Hori S;Tatsumi Y;Onishi S;Owari T;Iida K;Onishi K;Gotoh D;Nakai Y;Anai S;Chihara Y;Torimoto K;Aoki K;Tanaka N;Shimada K;Konishi N;Fujimoto K
由尿路上皮癌细胞产生的胶原蛋白4型α 1（COL 4A 1）和胶原蛋白13型α 1（COL 13 A1）支持肿瘤侵袭的重要致癌特性。我们研究了尿液中COL 4A 1和COL 13 A1的诊断和预后能力，并将观察值与细胞角蛋白-19片段（CYFRA 21 - 1），核基质蛋白22（NMP-22）和膀胱癌（BCa）的尿液细胞学进行了比较。我们收集了154例新诊断为BCa的患者手术前和61例对照受试者的尿液样本。使用酶联免疫吸附试验测定尿液上清液中COL 4A 1、COL 13 A1、CYFRA 21 - 1和NMP-22的蛋白水平。通过受试者工作特征分析确定诊断性能和最佳临界值。与对照组相比，BCa患者尿液中的COL 4A 1、COL 13 A1、COL 4A 1和COL 13 A1的组合值（COL 4A 1 + COL 13 A1）和CYFRA 21 - 1水平显著升高。在这些生物标志物中，COL 4A 1 + COL 13 A1的最佳临界值为1.33 ng/mL，对低级别肿瘤、高级别肿瘤、Ta、T1和肌肉浸润性疾病的灵敏度分别为57.4%、83.7%、56.1%、80.7%和91.7%。我们评估了130例初次经尿道手术后非肌层浸润性BCa样本的术前尿液水平的预后价值。高尿COL 4A 1 + COL 13 A1被认为是膀胱内复发的独立危险因素。虽然这些数据需要外部验证，但尿COL 4A 1和COL 13 A1可能是BCa的潜在诊断和预后生物标志物。这种易于使用的尿液特征识别了非肌层浸润性和肌层浸润性BCa复发和进展概率较高的患者亚组。
Collagen type 4 alpha 1 (COL4A1) and collagen type 13 alpha 1 (COL13A1) produced by urothelial cancer cells support the vital oncogenic property of tumor invasion. We investigated the diagnostic and prognostic capability of COL4A1 and COL13A1 in voided urine and compared the observed values with those of fragments of cytokeratin‐19 (CYFRA21‐1), nuclear matrix protein 22 (NMP‐22), and voided urine cytology in bladder cancer (BCa). We collected voided urine samples from 154 patients newly diagnosed with BCa, before surgery and from 61 control subjects. Protein levels of COL4A1, COL13A1, CYFRA21‐1, and NMP‐22 in urine supernatants were measured using enzyme‐linked immunosorbent assays. Diagnostic performance and optimal cut‐off values were determined by receiver operating characteristic analysis. Urine levels of COL4A1, COL13A1, the combined values of COL4A1 and COL13A1 (COL4A1 + COL13A1), and CYFRA21‐1 were significantly elevated in urine from patients with BCa compared to the controls. Among these biomarkers, the optimal cut‐off value of COL4A1 + COL13A1 at 1.33 ng/mL resulted in 57.4%, 83.7%, 56.1%, 80.7%, and 91.7% sensitivity for low‐grade tumors, high‐grade tumors, Ta, T1, and muscle invasive disease, respectively. We evaluated the prognostic value of preoperative urine levels in 130 non‐muscle invasive BCa samples after the initial transurethral surgery. A high urinary COL4A1 + COL13A1 was found to be an independent risk factor for intravesical recurrence. Although these data need to be externally validated, urinary COL4A1 and COL13A1 could be a potential diagnostic and prognostic biomarker for BCa. This easy‐to‐use urinary signature identifies a subgroup of patients with a high probability of recurrence and progression in non‐muscle invasive and muscle invasive BCa.
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影响因子：
--
作者：
Miyake M;Hori S;Morizawa Y;Tatsumi Y;Toritsuka M;Ohnishi S;Shimada K;Furuya H;Khadka VS;Deng Y;Ohnishi K;Iida K;Gotoh D;Nakai Y;Inoue T;Anai S;Torimoto K;Aoki K;Tanaka N;Konishi N;Fujimoto K
通讯作者：
Fujimoto K
影响因子：
1.6
作者：
Lalkhen, Abdul Ghaaliq;McCluskey, Anthony
通讯作者：
McCluskey, Anthony
影响因子：
2
作者：
Miyake M;Goodison S;Giacoia EG;Rizwani W;Ross S;Rosser CJ
通讯作者：
Rosser CJ
影响因子：
4.8
作者：
Hagg, P;Rehn, M;Pihlajaniemi, T
通讯作者：
Pihlajaniemi, T
影响因子：
23.4
作者：
Sözen, S;Biri, H;Bozkirli, I
通讯作者：
Bozkirli, I