Improved thermal cycling durability and PCR compatibility of polymer coated quantum dot
Improved thermal cycling durability and PCR compatibility of polymer coated quantum dot
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提高聚合物涂层量子点的热循环耐久性和 PCR 兼容性
DOI：
10.1088/0957-4484/24/35/355504
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发表时间：
2013-08
期刊：
影响因子：
3.5
通讯作者：
Guan, Yifu
中科院分区：
文献类型：
--
作者：
Xun, Zhe;Zhao, Xiaoyun;Guan, Yifu
Quantum dots have experienced rapid development in imaging, labeling and sensing in medicine and life science. To be suitable for polymerase chain reaction (PCR) assay, we have tested QD thermal cycling durability and compatibility, which have not been addressed in previous reports. In this study, we synthesized CdSe/ZnS QDs with a surface modification with high-MW amphiphilic copolymers and observed that Mg2+ ions in the PCR reaction could induce the QDs to precipitate and reduce their fluorescence signal significantly after thermal cycling. To overcome this problem, we used mPEG2000 to conjugate the QD surface for further protection, and found that this modification enables QDs to endure 40 thermal cycles in the presence of other components essential for PCR reactions. We have also identified that QDs have different effects on rTaq and Ex Taq polymerization systems. A high QD concentration could apparently reduce the PCR efficiency, but this inhibition was relieved significantly in the Ex PCR system as the concentration of Ex Taq polymerase was increased. Real-time PCR amplification results showed that QDs could provide a sufficiently measurable fluorescence signal without excessively inhibiting the DNA amplification. Based on this improved thermal cycling durability and compatibility with the PCR system, QDs have the potential to be developed as stable fluorescent sensors in PCR and real-time PCR amplification.
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影响因子：
17.1
作者：
Prasuhn DE;Blanco-Canosa JB;Vora GJ;Delehanty JB;Susumu K;Mei BC;Dawson PE;Medintz IL
通讯作者：
Medintz IL
影响因子：
--
作者：
Liang, Gaofeng;Ma, Chao;Zhu, Yanliang;Li, Shuchun;Shao, Youhua;Wang, Yong;Xiao, Zhongdang
通讯作者：
Xiao, Zhongdang
影响因子：
17.1
作者：
K. Sapsford;Jessica Granek;J. Deschamps;K. Boeneman;J. Blanco-Canosa;P. Dawson;K. Susumu;M. Stewart;Igor L. Medintz
通讯作者：
K. Sapsford;Jessica Granek;J. Deschamps;K. Boeneman;J. Blanco-Canosa;P. Dawson;K. Susumu;M. Stewart;Igor L. Medintz
影响因子：
17.1
作者：
Anderson, Robin E.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
17.1
作者：
Hu X;Gao X
通讯作者：
Gao X