Identification of an Endophytic Antifungal Bacterial Strain Isolated from the Rubber Tree and Its Application in the Biological Control of Banana Fusarium Wilt.
Identification of an Endophytic Antifungal Bacterial Strain Isolated from the Rubber Tree and Its Application in the Biological Control of Banana Fusarium Wilt.
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橡胶树内生抗真菌菌株的鉴定及其在香蕉枯萎病生物防治中的应用
DOI：
10.1371/journal.pone.0131974
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Tan D;Fu L;Han B;Sun X;Zheng P;Zhang J
香蕉枯萎病（又称巴拿马病）是危害最严重的植物病害之一。有效的控制方法仍在探索中。从橡胶树中分离到一株内生细菌ITBB B5-1，经形态学、生化和系统发育分析，鉴定为粘质沙雷氏菌（Serratia marcescens）。该菌株对香蕉镰刀菌病具有很高的生物防治潜力。琼脂平板试验表明，ITBB B5-1对黄瓜枯萎病菌菌丝生长有明显的抑制作用。古巴小种4号（FOC 4）。显微镜观察发现，靠近共培养细菌的FOC 4菌丝细胞壁部分分解，分生孢子的形成受到抑制。经茎尖培养法测定，ITBB B5-1菌株培养液对病原真菌的抑制率为95.4%。在培养液中检测到几丁质酶和葡聚糖酶活性，并且几丁质酶和葡聚糖酶分别在孵育的第2天和第3天获得最高活性。过滤后的无细胞培养液降解了FOC 4菌丝体的细胞壁。这些结果表明，几丁质酶和葡聚糖酶参与了ITBB B5-1的抗真菌机制。在绿色室实验中，在用FOC 4感染之前用ITBB B5-1接种的盆栽香蕉植物显示出78.7%的疾病严重度指数降低。在田间试验中，ITBB B5-1对该病的防治效果约为70.0%。因此，内生细菌ITBB B5-1可用于香蕉枯萎病的生物防治。
Banana Fusarium wilt (also known as Panama disease) is one of the most disastrous plant diseases. Effective control methods are still under exploring. The endophytic bacterial strain ITBB B5-1 was isolated from the rubber tree, and identified as Serratia marcescens by morphological, biochemical, and phylogenetic analyses. This strain exhibited a high potential for biological control against the banana Fusarium disease. Visual agar plate assay showed that ITBB B5-1 restricted the mycelial growth of the pathogenic fungus Fusarium oxysporum f. sp. cubense race 4 (FOC4). Microscopic observation revealed that the cell wall of the FOC4 mycelium close to the co-cultured bacterium was partially decomposed, and the conidial formation was prohibited. The inhibition ratio of the culture fluid of ITBB B5-1 against the pathogenic fungus was 95.4% as estimated by tip culture assay. Chitinase and glucanase activity was detected in the culture fluid, and the highest activity was obtained at Day 2 and Day 3 of incubation for chitinase and glucanase, respectively. The filtrated cell-free culture fluid degraded the cell wall of FOC4 mycelium. These results indicated that chitinase and glucanase were involved in the antifungal mechanism of ITBB B5-1. The potted banana plants that were inoculated with ITBB B5-1 before infection with FOC4 showed 78.7% reduction in the disease severity index in the green house experiments. In the field trials, ITBB B5-1 showed a control effect of approximately 70.0% against the disease. Therefore, the endophytic bacterial strain ITBB B5-1 could be applied in the biological control of banana Fusarium wilt.
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影响因子：
1.8
作者：
Huang YH;Wang RC;Li CH;Zuo CW;Wei YR;Zhang L;Yi GJ
通讯作者：
Yi GJ
影响因子：
4.5
作者：
Hwang, SC;Ko, WH
通讯作者：
Ko, WH
影响因子：
--
作者：
Forsyth, Leanne M.;Smith, Linda J.;Aitken, Elizabeth A. B.
通讯作者：
Aitken, Elizabeth A. B.
影响因子：
4.5
作者：
Bruton, BD;Fletcher, J;Sittertz-Bhatkar, H
通讯作者：
Sittertz-Bhatkar, H
影响因子：
2.1
作者：
Cao, LX;Qiu, ZQ;Zhou, SN
通讯作者：
Zhou, SN