Transcriptome-based gene expression profiling identifies differentially expressed genes critical for salt stress response in radish (Raphanus sativus L.)
Transcriptome-based gene expression profiling identifies differentially expressed genes critical for salt stress response in radish (Raphanus sativus L.)
复制标题
基于转录组的基因表达谱鉴定了对萝卜（Raphanus sativus L.）盐胁迫反应至关重要的差异表达基因
DOI：
10.1007/s00299-015-1887-5
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发表时间：
2016-02
影响因子：
6.2
通讯作者：
Liu Liwang
中科院分区：
文献类型：
--
作者：
Sun Xiaochuan;Xu Liang;Wang Yan;Luo Xiaobo;Zhu Xianwen;Kinuthia Karanja Benard;Nie Shanshan;Feng Haiyang;Li Chao;Liu Liwang
基于转录组的基因表达分析发现了萝卜中许多关键的盐响应基因，有助于进一步剖析盐胁迫响应的分子机制。盐胁迫严重影响植物的生长发育。萝卜是一种对盐中等敏感的蔬菜作物，对其在盐胁迫下的生理生化表现进行了数十年的研究。然而，目前还没有对萝卜盐胁迫反应相关基因的分离和鉴定进行系统的研究，调控这一过程的分子机制也仍然不清楚。本研究应用RNA-Seq技术分析了盐(200 MM NaC L)处理萝卜根48h后与正常培养条件下萝卜根转录水平的变化。共鉴定出差异表达基因8709条，其中上调基因3931条，下调基因4778条。功能注释分析表明，许多基因可能参与盐胁迫反应的多个方面，包括胁迫感知和信号转导、渗透调节、离子动态平衡和ROS清除。盐响应基因与miRNAs的关联分析表明，36对miRNA-mRNAs在表达趋势上呈负相关。逆转录定量聚合酶链式反应(RT-qPCR)分析表明，DEGS的表达谱与RNA-Seq分析结果一致。此外，还提出了DGS和miRNA介导的基因调控模型，以阐明萝卜对盐胁迫的感知和响应。本研究首次提出了基于转录组的萝卜盐胁迫基因表达谱研究。本研究结果有助于进一步剖析盐胁迫反应的分子机制，为萝卜育种中进一步开展耐盐性遗传改良提供有价值的平台。
Transcriptome-based gene expression analysis identifies many critical salt-responsive genes in radish and facilitates further dissecting the molecular mechanism underlying salt stress response. Salt stress severely impacts plant growth and development. Radish, a moderately salt-sensitive vegetable crop, has been studied for decades towards the physiological and biochemical performances under salt stress. However, no systematic study on isolation and identification of genes involved in salt stress response has been performed in radish, and the molecular mechanism governing this process is still indistinct. Here, the RNA-Seq technique was applied to analyze the transcriptomic changes on radish roots treated with salt (200 mM NaCl) for 48 h in comparison with those cultured in normal condition. Totally 8709 differentially expressed genes (DEGs) including 3931 up- and 4778 down-regulated genes were identified. Functional annotation analysis indicated that many genes could be involved in several aspects of salt stress response including stress sensing and signal transduction, osmoregulation, ion homeostasis and ROS scavenging. The association analysis of salt-responsive genes and miRNAs exhibited that 36 miRNA-mRNA pairs had negative correlationship in expression trends. Reverse-transcription quantitative PCR (RT-qPCR) analysis revealed that the expression profiles of DEGs were in line with results from the RNA-Seq analysis. Furthermore, the putative model of DEGs and miRNA-mediated gene regulation was proposed to elucidate how radish sensed and responded to salt stress. This study represents the first comprehensive transcriptome-based gene expression profiling under salt stress in radish. The outcomes of this study could facilitate further dissecting the molecular mechanism underlying salt stress response and provide a valuable platform for further genetic improvement of salt tolerance in radish breeding programs.
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影响因子：
4.4
作者：
Sun X;Xu L;Wang Y;Yu R;Zhu X;Luo X;Gong Y;Wang R;Limera C;Zhang K;Liu L
通讯作者：
Liu L
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
DOI：
10.1007/978-1-4939-2211-6_8
发表时间：
2015
期刊：
--
影响因子：
--
作者：
H. Ara;A. Sinha
通讯作者：
H. Ara;A. Sinha
影响因子：
3.7
作者：
Liu H;Kohane IS
通讯作者：
Kohane IS
GHSOD1和GHCAT1过表达在棉叶绿体中的协同作用对增强对甲基元素和盐胁迫的耐受性。
影响因子：
3.7
作者：
Luo X;Wu J;Li Y;Nan Z;Guo X;Wang Y;Zhang A;Wang Z;Xia G;Tian Y
通讯作者：
Tian Y