Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.).
Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.).
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DOI：
10.1186/s12864-015-1416-5
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发表时间：
2015-03-17
期刊：
影响因子：
4.4
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Sun X;Xu L;Wang Y;Yu R;Zhu X;Luo X;Gong Y;Wang R;Limera C;Zhang K;Liu L
盐胁迫是最具代表性的非生物胁迫之一，严重影响植物的生长发育。MicroRNAs（miRNAs）是一类重要的参与植物对非生物胁迫响应的分子。尽管在许多植物物种中已经广泛报道了与盐胁迫反应有关的miRNAs，但它们在萝卜（Raphanus sativus L.）对盐胁迫的适应性反应中的调节作用，一种世界范围内重要的根菜作物，在很大程度上仍不为人知。对无NaCl（CK）和NaCl处理（Na 200）萝卜根的sRNA文库进行Solexa测序，以系统鉴定萝卜盐响应性miRNAs及其表达谱。共鉴定出136个已知的miRNA（代表43个miRNA家族）和68个潜在的新miRNA（属于51个miRNA家族）。在这些miRNAs中，49个已知的和22个新的miRNAs在盐胁迫下差异表达。靶点预测和注释表明，这些miRNAs通过调控特定的胁迫应答基因发挥作用，如鳞状细胞启动子结合样蛋白（SPLs）、生长素应答因子（ARF）、核转录因子Y（NF-Y）和超氧化物歧化酶[Cu-Zn]（CSD 1）。进一步的功能分析表明，这些靶基因主要参与信号的感知和转导、离子稳态的调节、基本代谢过程、次生胁迫反应以及盐胁迫下植物生长发育的调控。此外，通过逆转录定量PCR（RT-qPCR）验证了10种miRNAs和5种相应靶基因的表达模式。通过sRNA测序，全面鉴定了萝卜盐响应miRNAs及其靶基因。这些结果为研究萝卜盐胁迫反应中miRNA介导的复杂调控网络提供了新的视角，有助于进一步揭示根菜类作物盐胁迫适应性反应的分子机制。本文的在线版本（doi：10.1186/s12864-015-1416-5）包含补充材料，可供授权用户使用。
Salt stress is one of the most representative abiotic stresses that severely affect plant growth and development. MicroRNAs (miRNAs) are well known for their significant involvement in plant responses to abiotic stresses. Although miRNAs implicated in salt stress response have been widely reported in numerous plant species, their regulatory roles in the adaptive response to salt stress in radish (Raphanus sativus L.), an important root vegetable crop worldwide, remain largely unknown. Solexa sequencing of two sRNA libraries from NaCl-free (CK) and NaCl-treated (Na200) radish roots were performed for systematical identification of salt-responsive miRNAs and their expression profiling in radish. Totally, 136 known miRNAs (representing 43 miRNA families) and 68 potential novel miRNAs (belonging to 51 miRNA families) were identified. Of these miRNAs, 49 known and 22 novel miRNAs were differentially expressed under salt stress. Target prediction and annotation indicated that these miRNAs exerted a role by regulating specific stress-responsive genes, such as squamosa promoter binding-like proteins (SPLs), auxin response factors (ARFs), nuclear transcription factor Y (NF-Y) and superoxide dismutase [Cu-Zn] (CSD1). Further functional analysis suggested that these target genes were mainly implicated in signal perception and transduction, regulation of ion homeostasis, basic metabolic processes, secondary stress responses, as well as modulation of attenuated plant growth and development under salt stress. Additionally, the expression patterns of ten miRNAs and five corresponding target genes were validated by reverse-transcription quantitative PCR (RT-qPCR). With the sRNA sequencing, salt-responsive miRNAs and their target genes in radish were comprehensively identified. The results provide novel insight into complex miRNA-mediated regulatory network of salt stress response in radish, and facilitate further dissection of molecular mechanism underlying plant adaptive response to salt stress in root vegetable crops. The online version of this article (doi:10.1186/s12864-015-1416-5) contains supplementary material, which is available to authorized users.
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