Genotyping Cryptosporidium andersoni in cattle in Shaanxi Province, Northwestern China.
Genotyping Cryptosporidium andersoni in cattle in Shaanxi Province, Northwestern China.
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中国西北陕西省牛的安氏隐孢子虫基因分型
DOI：
10.1371/journal.pone.0060112
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang LX
中科院分区：
文献类型：
--
作者：
Zhao GH;Ren WX;Gao M;Bian QQ;Hu B;Cong MM;Lin Q;Wang RJ;Qi M;Qi MZ;Zhu XQ;Zhang LX
本研究调查了陕西省牛安氏隐孢子虫的流行情况和基因型。对2071份粪样（秦川牛847份，奶牛1224份）进行了隐孢子虫卵囊的检测，其中隐孢子虫卵囊70份（3.4%）。andersoni阳性和阴性标本分别用PCR扩增小亚基核糖体RNA（SSU rRNA）和隐孢子虫卵囊壁蛋白（COWP）基因进行鉴定。C. andersoni是该省检查的牛中唯一发现的物种。五十七度利用多位点序列分型技术将andersoni分离株分为5个MLST亚型，其中包括一个在地方肉牛品种秦川牛中发现的新亚型。所有这些C。andersoni分离物呈现克隆遗传结构。这些发现为C.对陕西省隐孢子虫病的流行现状进行了调查，为陕西省隐孢子虫病的防治提供了依据。
The present study examined the prevalence and genotypes of Cryptosporidium andersoni in cattle in Shaanxi province, China. A total of 2071 fecal samples (847 from Qinchuan cattle and 1224 from dairy cattle) were examined for the presence of Cryptosporidium oocysts, and 70 samples (3.4%) were C. andersoni-positive and those positive samples were identified by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) and the Cryptosporidium oocyst wall protein (COWP) genes. C. andersoni was the only species found in the examined cattle in this province. Fifty-seven C. andersoni isolates were characterized into 5 MLST subtypes using multilocus sequence typing analysis, including a new subtype in the native beef breed Qinchuan cattle. All of these C. andersoni isolates presented a clonal genetic structure. These findings provide new insights into the genetic structure of C. andersoni isolates in Shaanxi province and basic data of Cryptosporidium prevalence status, which in turn have implications for controlling cryptosporidiosis in this province.
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