Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells.
Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells.
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DOI：
10.1038/s41598-020-71567-w
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发表时间：
2020-09-18
影响因子：
4.6
通讯作者：
Ihalainen TO
中科院分区：
文献类型：
--
作者：
Fedele C;Mäntylä E;Belardi B;Hamkins-Indik T;Cavalli S;Netti PA;Fletcher DA;Nymark S;Priimagi A;Ihalainen TO
表面形貌是调节单细胞形态和行为的关键参数。在多细胞水平，协调的细胞位移驱动许多生物事件，如胚胎形态发生。然而，尚未详细研究表面形貌对上皮集体迁移的影响。掌握表面特征和集体细胞行为之间的联系对于组织工程和修复的新方法非常重要。在此，我们使用了含偶氮苯的材料上的微形貌，并表明具有适当周期和方向的平滑形貌线索可以有效地协调生长上皮中的细胞排列。此外，该实验系统使我们能够研究地形特征的方向如何改变体外伤口闭合的速度。我们的研究结果表明，细胞外微环境的地形协调他们的焦点粘附分布和对齐。这些地形线索能够引导多细胞系统的集体迁移，即使细胞间连接被破坏。
Surface topography is a key parameter in regulating the morphology and behavior of single cells. At multicellular level, coordinated cell displacements drive many biological events such as embryonic morphogenesis. However, the effect of surface topography on collective migration of epithelium has not been studied in detail. Mastering the connection between surface features and collective cellular behaviour is highly important for novel approaches in tissue engineering and repair. Herein, we used photopatterned microtopographies on azobenzene-containing materials and showed that smooth topographical cues with proper period and orientation can efficiently orchestrate cell alignment in growing epithelium. Furthermore, the experimental system allowed us to investigate how the orientation of the topographical features can alter the speed of wound closure in vitro. Our findings indicate that the extracellular microenvironment topography coordinates their focal adhesion distribution and alignment. These topographic cues are able to guide the collective migration of multicellular systems, even when cell–cell junctions are disrupted.
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影响因子：
3.4
作者：
Besser, A;Safran, SA
通讯作者：
Safran, SA
影响因子：
19.6
作者：
Brugués A;Anon E;Conte V;Veldhuis JH;Gupta M;Colombelli J;Muñoz JJ;Brodland GW;Ladoux B;Trepat X
通讯作者：
Trepat X
影响因子：
3.9
作者：
Clemons, T. D.;Bradshaw, M.;Toshniwal, P.;Chaudhari, N.;Stevenson, A. W.;Lynch, J.;Fear, M. W.;Wood, F. M.;Iyer, K. Swaminathan
通讯作者：
Iyer, K. Swaminathan
DOI：
10.1126/science.1176009
发表时间：
2009-11-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hynes RO
通讯作者：
Hynes RO
影响因子：
4.9
作者：
Diehl, KA;Foley, JD;Murphy, CJ
通讯作者：
Murphy, CJ