Normal Table of Xenopus development: a new graphical resource.

Normal Table of Xenopus development: a new graphical resource.
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DOI:
10.1242/dev.200356
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发表时间:
2022-07-15
期刊:
Development (Cambridge, England)
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其他
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正常的发育表对于胚胎发生的研究是必不可少的,是包括非洲爪蟾在内的模式生物的重要资源。非洲爪蟾长期以来一直被用于研究发育和细胞生物学,并且是人类出生缺陷和疾病,基因组学,蛋白质组学和毒理学的重要模型。科学家们利用Nieuwkoop和Faber的经典“非洲爪蟾(Daudin)的正常表”和随附的插图,使实验的可重复性和重复使用的插图在新的出版物和教学。然而,现在已经不可能获得这些受版权保护的插图的许可。我们提出了133个新的,高品质的X插图。laevis从受精到变态的发展,以及原始收藏中没有的其他观点。所有的图像都可以在Xenbase(Xenopus knowledgebase)上获得,可以在可归属的、非商业性的创作共用许可证下下载和重用。此外,我们还编制了一个关键形态特征和标记基因表达的“地标表”,可用于快速可靠地区分阶段()。这个新的开放获取资源将在未来几十年内促进非洲爪蟾的研究和教学。总结:一个新的开放获取的正常表爪蟾的发展,集成了新的胚胎图像与基因表达标记在Xenbase,爪蟾生物医学研究知识库。
Normal tables of development are essential for studies of embryogenesis, serving as an important resource for model organisms, including the frog Xenopus laevis. Xenopus has long been used to study developmental and cell biology, and is an increasingly important model for human birth defects and disease, genomics, proteomics and toxicology. Scientists utilize Nieuwkoop and Faber's classic ‘Normal Table of Xenopus laevis (Daudin)’ and accompanying illustrations to enable experimental reproducibility and reuse the illustrations in new publications and teaching. However, it is no longer possible to obtain permission for these copyrighted illustrations. We present 133 new, high-quality illustrations of X. laevis development from fertilization to metamorphosis, with additional views that were not available in the original collection. All the images are available on Xenbase, the Xenopus knowledgebase (), for download and reuse under an attributable, non-commercial creative commons license. Additionally, we have compiled a ‘Landmarks Table’ of key morphological features and marker gene expression that can be used to distinguish stages quickly and reliably (). This new open-access resource will facilitate Xenopus research and teaching in the decades to come. Summary: A new open-access Normal Table of Xenopus development is presented that integrates new embryo images with gene expression markers in Xenbase, the Xenopus biomedical research knowledgebase.
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