Molecular Characterization Based on MLST and ECDC Typing Schemes and Antibiotic Resistance Analyses of Treponema pallidum subsp. pallidum in Xiamen, China.
Molecular Characterization Based on MLST and ECDC Typing Schemes and Antibiotic Resistance Analyses of Treponema pallidum subsp. pallidum in Xiamen, China.
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基于 MLST 和 ECDC 分型方案的中国厦门苍白链球菌亚种的分子特征和抗生素耐药性分析。
DOI：
10.3389/fcimb.2020.618747
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发表时间：
2020
影响因子：
5.7
通讯作者：
Yang TC
中科院分区：
文献类型：
--
作者：
Liu D;He SM;Zhu XZ;Liu LL;Lin LR;Niu JJ;Yang TC
共分析了49份临床样本，使用两种分型方案，增强的疾病控制和预防中心（ECDC）和多位点序列分型（MLST），以描述2016年至2017年厦门市梅毒螺旋体循环分离株的分子特征。此外，还发现了与T.苍白球也进行了分析。45份样本经ECDC全分型，共检出14种不同亚型。最常见的亚型为16 d/f（24.4%），其次为14 d/f（20.0%）。所有49个样本成功分型MLST，而只有四个等位基因的配置文件，包括三个SS 14样的配置文件和一个尼科尔斯样的配置文件。主要等位基因频率为1.1.8（85.7%）。有趣的是，在欧洲和北美广泛分布的等位基因谱1.3.1在该地区没有检测到。此外，所有可检测样本中均发现23 S rRNA的A2058 G突变（38/38），未观察到16 S rRNA的突变（36/36）。在符合桑格测序条件的35个样本中发现了青霉素结合蛋白基因的4个非同义单核苷酸多态性。其中tp 0500（P564 I）的变异仅见于SS 14样株。tp 0705（A506 V/A506 T）和tp 0760（I415 F/I415 M）基因组中存在同源性变异，并且在SS 14样菌株和Nichols样菌株中分别出现了tp 0705 A506 V和tp 0705 A506 T变异。本研究表明，T. 2016 - 2017年厦门地区的梅毒螺旋体分离株与其他地理区域的分离株不同。T.本研究中鉴定的梅毒分离株可为临床医生提供梅毒治疗的认识。
In total, 49 clinical samples were analyzed using two typing schemes, Enhanced Centers for Disease Control and Prevention (ECDC) and multilocus sequence typing (MLST), to describe the molecular characteristics of circulating Treponema pallidum isolates in Xiamen between 2016 and 2017. In addition, genetic mutations potentially related to antibiotic resistance of T. pallidum were also analyzed. Forty five samples were fully typed by ECDC, and 14 different subtypes were detected. The most common subtype was 16d/f (24.4%), followed by 14d/f (20.0%). All forty nine samples were successfully typed by MLST, while only four allelic profiles were identified, including three SS14-like profiles and one Nichols-like profile. Among them, the major allelic profile was 1.1.8 (85.7%). Interestingly, the allelic profile 1.3.1 widespread in Europe and North America was not detected in this region. Additionally, A2058G mutation in 23S rRNA was found in all detectable samples (38/38), and no mutation in 16S rRNA was observed (36/36). Four non-synonymous single-nucleotide polymorphisms in penicillin-binding protein genes were found in the 35 samples eligible for Sanger sequencing. Among them, the variant in tp0500 (P564I) can only be found in the SS14-like isolates. Homoplastic changes in tp0760 (I415F/I415M) and tp0705 (A506V/A506T) were found. Moreover, the variant tp0705 A506V and the variant tp0705 A506T separately appeared in the SS14-like isolates and Nichols-like isolates, respectively. This study showed that the genotypes of T. pallidum isolates in Xiamen between 2016 and 2017 were different from those in other geographic areas. The resistance-related variants of T. pallidum isolates identified in this study could provide awareness for clinicians in the treatment of syphilis.
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影响因子：
3.7
作者：
Gallo Vaulet L;Grillová L;Mikalová L;Casco R;Rodríguez Fermepin M;Pando MA;Šmajs D
通讯作者：
Šmajs D
利用下一代测序追踪中国梅毒螺旋体的起源
影响因子：
--
作者：
Sun J;Meng Z;Wu K;Liu B;Zhang S;Liu Y;Wang Y;Zheng H;Huang J;Zhou P
通讯作者：
Zhou P
影响因子：
9.4
作者：
Dai, Ting;Li, Kang;Zhou, Pingyu
通讯作者：
Zhou, Pingyu
影响因子：
3.6
作者：
Flasarova, Magdalena;Pospisilova, Petra;Smajs, David
通讯作者：
Smajs, David
DOI：
10.1086/656533
发表时间：
2010-11-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Marra C;Sahi S;Tantalo L;Godornes C;Reid T;Behets F;Rompalo A;Klausner JD;Yin Y;Mulcahy F;Golden MR;Centurion-Lara A;Lukehart SA
通讯作者：
Lukehart SA