Enhanced molecular typing of treponema pallidum: geographical distribution of strain types and association with neurosyphilis.
Enhanced molecular typing of treponema pallidum: geographical distribution of strain types and association with neurosyphilis.
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DOI：
10.1086/656533
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发表时间：
2010-11-01
期刊：
影响因子：
--
通讯作者：
Lukehart SA
中科院分区：
文献类型：
--
作者：
Marra C;Sahi S;Tantalo L;Godornes C;Reid T;Behets F;Rompalo A;Klausner JD;Yin Y;Mulcahy F;Golden MR;Centurion-Lara A;Lukehart SA
菌株分型是确定感染多样性和流行病学的一种工具。从美国、中国、爱尔兰和马达加斯加的158例梅毒患者和15株梅毒T. pallidum分离株中分离出梅毒T. pallidum DNA。评估了6个分型目标：1)酸性重复蛋白基因60 bp重复数；2) T. pallidum repeat （tpr）亚家族II基因限制性片段长度多态性（RFLP）分析；3) tprC基因的RFLP分析；4) tprD基因座等位基因的测定；5) tp0126/tp0127之间存在51 bp的插入；6) tp0548基因84bp区序列分析。#1和#2的组合构成了CDC苍白球绦虫亚型分型方法。将tp0548的序列分析加入CDC方法中，得到了最具鉴别性的分型系统。共鉴定出24种菌株，并确定为CDC亚型/tp0548序列。6个位置中有5个可见14d/f型。在西雅图，菌株类型从1999年到2008年发生了变化（p<0.001）。44例14d/f型感染患者中有22例（50%）发生神经梅毒，39例其他类型合并感染的患者中有9例（23%）发生神经梅毒（p=0.01）。我们描述了一种增强的苍白球绦虫菌株分型系统，显示生物学和临床相关性。
Strain typing is a tool for determining diversity and epidemiology of infections. T. pallidum DNA was isolated from 158 syphilis patients from the US, China, Ireland, and Madagascar and from 15 T. pallidum isolates. Six typing targets were assessed: 1) number of 60 bp repeats in acidic repeat protein gene; 2) restriction fragment length polymorphism (RFLP) analysis of T. pallidum repeat (tpr) subfamily II genes; 3) RFLP analysis of tprC gene; 4) determination of tprD allele in tprD gene locus; 5) presence of 51 bp insertion between tp0126/tp0127; 6) sequence analysis of 84 bp region of tp0548. The combination of #1 and #2 comprises the CDC T. pallidum subtyping method. Adding sequence analysis of tp0548 to the CDC method yielded the most discriminating typing system. Twenty-four strain types were identified and designated as CDC subtype/tp0548 sequence. Type 14d/f was seen in 5 of 6 locations. In Seattle, strain types changed from 1999– 2008 (p<0.001). Twenty-two (50%) of 44 patients infected with type 14d/f had neurosyphilis compared to 9 (23%) of 39 infected with the other types combined (p=0.01). We describe an enhanced T. pallidum strain typing system that shows biological and clinical relevance.
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影响因子：
9.4
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