Assessing nutritional and genetic variations within foxtail millet (Setaria italica) landraces collected from indigenous communities across the Philippines.
Assessing nutritional and genetic variations within foxtail millet (Setaria italica) landraces collected from indigenous communities across the Philippines.
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DOI：
10.1016/j.heliyon.2023.e22964
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发表时间：
2023-12
期刊：
影响因子：
4
通讯作者：
Evelyn F. Delfin
中科院分区：
文献类型：
--
作者：
Nelzo C. Ereful;Jose Arnel O. Reyes;Don Emanuel M. Cardona;Roneil Christian S. Alonday;Nel Oliver B. Mateo;Huw Jones;Lesley Boyd;Evelyn F. Delfin
许多人都不知道，菲律宾有一些未被充分利用和研究的谷子（Setaria italica (L.)）的剩余品种。p .测定)。我们收集了来自菲律宾不同生态地理位置的这种作物的材料，以及来自台湾兰屿的一些材料，对其营养价值和遗传多样性进行了研究。所有材料均于2022年旱季在菲律宾拉古纳Los Baños植物育种研究所（IPB）实验站进行田间种植。对这些产品进行微量营养素测试，包括锌和铁，氮（蛋白质的替代品），β-胡萝卜素，植酸和一些已知营养潜力的酚类化合物。其中，巴亚拉斯和GB61438的锌含量最高（107.1 mg/kg），铁含量最高（70.52 mg/kg），高于传统水稻品种。其中β-胡萝卜素含量最高的是加入球（~ 10 μg/g）。共检出12种酚类化合物，其中儿茶素、丁香酸、阿魏酸和山奈酚含量最高。为了评估遗传多样性，我们使用全基因组重测序技术对8个样本进行了至少25倍深度的测序。群体结构分析，使用全基因组，高质量的snp，显示适度的多样性在加入，有两个未混合的群体。相对于它们最早的共同祖先，这些品种是单系的，浅棕色品种比浅棕色和红棕色品种出现得更早。谷子参考序列Yugu1中锌调控、铁调控转运蛋白（ZIP）转运蛋白的分析鉴定出17个可能的ZIP转运蛋白。变异呼叫主要在3 ‘和5 ’区域以及内含子中发现snp，表明谷子在调节基因区域而不是结构蛋白中存在差异。因此，当地的谷子产量代表了一种潜在的替代营养来源，可能有助于解决菲律宾的营养不良问题。
Unknown to many, the Philippines is host to a few remaining accessions of the underutilised and understudied cereal foxtail millet (Setaria italica (L.) P. Beauv.). We collected together accessions of this crop from different eco-geographical locations within the Philippines, along with a few accessions from Lanyu, Taiwan, to undertake a study of their nutritional value and genetic diversity. All accessions were field-grown in 2022, dry season (DS) at the Institute of Plant Breeding (IPB) Experiment Station, Los Baños, Laguna, Philippines. The accessions were tested for micronutrients, including Zn and Fe, nitrogen as a proxy for protein, β-carotene, phytic acid, and a number of phenolic compounds with known nutritional potential. Of the 20 accessions tested, the accessions Bayaras and GB61438 had the highest level of Zn (107.1 mg/kg) and Fe (70.52 mg/kg), respectively, higher than levels found in traditional rice varieties. For β-carotene the highest concentration was found in the accession Balles (∼10 μg/g). Twelve phenolic compounds were detected, with catechin, syringic acid, ferulic acid and kaempferol having the highest concentrations. To assess the genetic diversity, we sequenced a set of eight samples selected from among the accessions to a depth of at least 25-fold using whole-genome re-sequencing. Analysis of the population structure, using genome-wide, high-quality SNPs, showed modest diversity among the accessions, with two unadmixed groups. The accessions are monophyletic relative to their earliest common ancestor, with the very light brown accessions emerging earlier than the light brown and reddish-brown varieties. Analysis of zinc-regulated, iron-regulated transporter-like protein (ZIP) transporters within the foxtail millet reference sequence, var. Yugu1 identified 17 putative ZIP transporters. Variant calling identified SNPs primarily within 3′ and 5’ regions, and introns, indicating variation between foxtail millet accessions within regulatory gene regions rather than in structural proteins. The local foxtail millet accessions, therefore, represent a potential alternative source of nutrients which may help in addressing malnutrition in the Philippines.
影响因子：
5.3
作者：
Ereful, Nelzo C.;Jones, Huw;Fradgley, Nick;Boyd, Lesley;Cherie, Hirut Assaye;Milner, Matthew J.
通讯作者：
Milner, Matthew J.
影响因子：
4.6
作者：
Ereful NC;Laurena A;Liu LY;Kao SM;Tsai E;Greenland A;Powell W;Mackay I;Leung H
通讯作者：
Leung H