Unraveling regulatory divergence, heterotic malleability, and allelic imbalance switching in rice due to drought stress.
Unraveling regulatory divergence, heterotic malleability, and allelic imbalance switching in rice due to drought stress.
复制标题
DOI：
10.1038/s41598-021-92938-x
复制
发表时间：
2021-06-29
影响因子：
4.6
通讯作者：
Leung H
中科院分区：
文献类型：
--
作者：
Ereful NC;Laurena A;Liu LY;Kao SM;Tsai E;Greenland A;Powell W;Mackay I;Leung H
将适应灌溉生态系统的抗旱优良品种IR64和旱地中等抗旱基因型Apo （IR55423-01或NSIC RC9）及其杂交品种IR64 × Apo分别置于非胁迫和水分胁迫条件下。通过对这些基因型进行测序（RNA-seq），我们能够绘制出顺式和/或反式因子分化的基因图谱。在非应激条件下，顺式（cis）占主导地位（11.2%），其次是反式（8.9%）。进一步分析表明，限水条件对反式因子和顺式+反式因子影响较大。在分子水平上，顺式和/或反式调控差异解释了它们的基因型差异和不同的干旱反应。在两种亲本基因型之间，载脂蛋白即使在限水条件下也表现出更高的光合效率，并归因于反式。统计分析表明，监管差异受到环境条件的显著影响。同样，驱动杂种优势的亲本表达遗传模式（HET）也受到环境条件的显著影响，表明杂种优势对外部因素具有延展性。进一步分析表明，在水分胁迫条件下，HET类显著富集。我们还发现了等位基因的不平衡转换，在非胁迫条件下，一些基因倾向于IR64-（或载脂蛋白-）特异性等位基因，而在暴露于水分胁迫条件下，则转向载脂蛋白-（或IR64-）特异性等位基因。
The indica ecotypes, IR64, an elite drought-susceptible variety adapted to irrigated ecosystem, and Apo (IR55423-01 or NSIC RC9), a moderate drought-tolerant upland genotype together with their hybrid (IR64 × Apo) were exposed to non- and water-stress conditions. By sequencing (RNA-seq) these genotypes, we were able to map genes diverging in cis and/or trans factors. Under non-stress condition, cis dominantly explains (11.2%) regulatory differences, followed by trans (8.9%). Further analysis showed that water-limiting condition largely affects trans and cis + trans factors. On the molecular level, cis and/or trans regulatory divergence explains their genotypic differences and differential drought response. Between the two parental genotypes, Apo appears to exhibit more photosynthetic efficiency even under water-limiting condition and is ascribed to trans. Statistical analyses showed that regulatory divergence is significantly influenced by environmental conditions. Likewise, the mode of parental expression inheritance which drives heterosis (HET) is significantly affected by environmental conditions indicating the malleability of heterosis to external factors. Further analysis revealed that the HET class, dominance, was significantly enriched under water-stress condition. We also identified allelic imbalance switching in which several genes prefer IR64- (or Apo-) specific allele under non-stress condition but switched to Apo- (or IR64-) specific allele when exposed to water-stress condition.
登录
查看更多内容
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
11.6
作者：
Cubillos, Francisco A.;Stegle, Oliver;Loudet, Olivier
通讯作者：
Loudet, Olivier
DOI：
10.1186/s12284-016-0123-4
发表时间：
2016-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Ereful NC;Liu LY;Tsai E;Kao SM;Dixit S;Mauleon R;Malabanan K;Thomson M;Laurena A;Lee D;Mackay I;Greenland A;Powell W;Leung H
通讯作者：
Leung H
影响因子：
14.9
作者：
Alexandrov N;Tai S;Wang W;Mansueto L;Palis K;Fuentes RR;Ulat VJ;Chebotarov D;Zhang G;Li Z;Mauleon R;Hamilton RS;McNally KL
通讯作者：
McNally KL
影响因子：
56.9
作者：
Landry, Christian R.;Lemos, Bernardo;Hartl, Daniel L.
通讯作者：
Hartl, Daniel L.