Nutritional and genetic variation in a core set of Ethiopian Tef (Eragrostis tef) varieties.
Nutritional and genetic variation in a core set of Ethiopian Tef (Eragrostis tef) varieties.
复制标题
DOI：
10.1186/s12870-022-03595-9
复制
发表时间：
2022-04-28
影响因子：
5.3
通讯作者：
Milner, Matthew J.
中科院分区：
文献类型：
--
作者：
Ereful, Nelzo C.;Jones, Huw;Fradgley, Nick;Boyd, Lesley;Cherie, Hirut Assaye;Milner, Matthew J.
Tef（Eragrostis tef）是一种热带谷物，在埃塞俄比亚高地驯化和种植，几个世纪以来一直是埃塞俄比亚人的主食。粮食不安全和营养缺乏是该国的主要问题，因此培育增强营养性状，如锌含量，有助于缓解营养不良问题。为了了解tef营养性状的育种潜力，对24个核心品种进行了测序，并测量了它们的矿物质含量、植酸盐和蛋白质水平，以及谷物中一些有营养价值的酚类化合物。这些性状在品种间存在显著差异。24个tef品种的全基因组测序揭示了相对于tef参考变种的3，193，582个独特的SNP和897，272个独特的INDEL。达比对参与植物锌吸收和运输的两个关键转运蛋白家族进行序列分析，鉴定出32个锌铁渗透酶（ZIP）转运蛋白和14个重金属相关（HMA）转运蛋白。进一步分析发现了许多变体，其中14.6%的EtZIP和12.4%的EtHMA变体是非同义变化。对黄烷醇合成中的关键酶类黄酮3′-羟化酶（F3 'H）的分析，鉴定了tef同系物Et_s3159-0.29- 1.mrna 1中的T-G变体，其与观察到的山奈酚糖苷和槲皮素糖苷水平的差异相关。广泛的遗传和表型变异被发现在24个埃塞俄比亚茶品种，这将允许育种增益在许多重要的营养性状对人类健康。在线版本包含补充材料，可通过10.1186/s12870-022-03595-9获得。
Tef (Eragrostis tef) is a tropical cereal domesticated and grown in the Ethiopian highlands, where it has been a staple food of Ethiopians for many centuries. Food insecurity and nutrient deficiencies are major problems in the country, so breeding for enhanced nutritional traits, such as Zn content, could help to alleviate problems with malnutrition. To understand the breeding potential of nutritional traits in tef a core set of 24 varieties were sequenced and their mineral content, levels of phytate and protein, as well as a number of nutritionally valuable phenolic compounds measured in grain. Significant variation in all these traits was found between varieties. Genome wide sequencing of the 24 tef varieties revealed 3,193,582 unique SNPs and 897,272 unique INDELs relative to the tef reference var. Dabbi. Sequence analysis of two key transporter families involved in the uptake and transport of Zn by the plant led to the identification of 32 Zinc Iron Permease (ZIP) transporters and 14 Heavy Metal Associated (HMA) transporters in tef. Further analysis identified numerous variants, of which 14.6% of EtZIP and 12.4% of EtHMA variants were non-synonymous changes. Analysis of a key enzyme in flavanol synthesis, flavonoid 3′-hydroxylase (F3’H), identified a T-G variant in the tef homologue Et_s3159-0.29-1.mrna1 that was associated with the differences observed in kaempferol glycoside and quercetin glycoside levels. Wide genetic and phenotypic variation was found in 24 Ethiopian tef varieties which would allow for breeding gains in many nutritional traits of importance to human health. The online version contains supplementary material available at 10.1186/s12870-022-03595-9.
登录
查看更多内容
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
3.6
作者：
HEATHCOTE, JG
通讯作者：
HEATHCOTE, JG
影响因子：
3.1
作者：
Klein, Melinda A.;Grusak, Michael A.
通讯作者：
Grusak, Michael A.
影响因子：
4.3
作者：
Abebe, Yewelsew;Bogale, Alemtsehay;Gibson, Rosalind S.
通讯作者：
Gibson, Rosalind S.
DOI：
10.1073/pnas.95.12.7220
发表时间：
1998-06-09
影响因子：
11.1
作者：
Grotz, N;Fox, T;Eide, D
通讯作者：
Eide, D