Bacterial toxin-triggered drug release from gold nanoparticle-stabilized liposomes for the treatment of bacterial infection.
Bacterial toxin-triggered drug release from gold nanoparticle-stabilized liposomes for the treatment of bacterial infection.
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DOI：
10.1021/ja111110e
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发表时间：
2011-03-23
影响因子：
15
通讯作者：
Zhang, Liangfang
中科院分区：
文献类型：
--
作者：
Pornpattananangkul, Dissaya;Zhang, Li;Olson, Sage;Aryal, Santosh;Obonyo, Marygorret;Vecchio, Kenneth;Huang, Chun-Ming;Zhang, Liangfang
我们报告了一种新的方法，选择性地提供抗菌剂的细菌感染的网站，利用细菌毒素激活药物从金纳米颗粒稳定的磷脂脂质体释放。壳聚糖修饰的金纳米颗粒与脂质体表面的结合可以有效地防止它们彼此融合以及在常规储存或生理环境中不期望的有效载荷释放。然而，一旦这些受保护的脂质体“看到”分泌毒素的细菌，毒素将插入脂质体膜中并形成孔，包封的治疗剂通过孔释放。释放的药物随后对分泌毒素的细菌施加抗菌作用。使用耐甲氧西林金黄色葡萄球菌（MRSA）作为模型细菌和万古霉素作为模型抗MRSA抗生素，我们证明，合成的金纳米颗粒稳定的脂质体可以完全释放包封的万古霉素在24小时内在MRSA细菌的存在下，并导致抑制MRSA的生长有效的等量的万古霉素加载的脂质体（没有纳米颗粒稳定剂）和自由万古霉素。这种细菌毒素使药物从纳米颗粒稳定的脂质体中释放，为治疗细菌感染提供了一种新的、安全有效的方法。该技术可广泛应用于治疗由分泌成孔毒素的细菌引起的各种感染。
We report a new approach to selectively delivering antimicrobials to the sites of bacterial infections by utilizing bacterial toxins to activate drug release from gold nanoparticle-stabilized phospholipid liposomes. The binding of chitosan modified gold nanoparticles to the surface of liposomes can effectively prevent them from fusing with one another and from undesirable payload release in regular storage or physiological environments. However, once these protected liposomes “see” bacteria that secrete toxins, the toxins will insert into the liposome membranes and form pores, through which the encapsulated therapeutic agents are released. The released drugs subsequently impose antimicrobial effects on the toxin-secreting bacteria. Using methicillin-resistant Staphycoccus aureus (MRSA) as a model bacterium and vacomycin as a model anti-MRSA antibiotic, we demonstrate that the synthesized gold nanoparticle-stabilized liposomes can completely release the encapsulated vacomycin within 24 h in the presence of MRSA bacteria and lead to inhibition of MRSA growth as effective as an equal amount of vacomycin loaded liposomes (without nanoparticle stabilizers) and free vacomycin. This bacterial toxin enabled drug release from nanoparticle-stabilized liposomes provides a new, safe and effective approach for the treatment of bacterial infections. This technique can be broadly applied to treat a variety of infections caused by bacteria that secrete pore-forming toxins.
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影响因子：
4.9
作者：
Hu CM;Kaushal S;Tran Cao HS;Aryal S;Sartor M;Esener S;Bouvet M;Zhang L
通讯作者：
Zhang L
DOI：
10.1016/j.saa.2005.04.048
发表时间：
2006-01-01
影响因子：
4.4
作者：
Aryal, S;Remant, BKC;Kim, HY
通讯作者：
Kim, HY
DOI：
10.1111/j.1432-1033.1989.tb14790.x
发表时间：
1989-05-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
FORTI, S;MENESTRINA, G
通讯作者：
MENESTRINA, G
影响因子：
4.1
作者：
Blackwood, RA;Smolen, JE;Transue, A
通讯作者：
Transue, A
影响因子：
3.6
作者：
STOCKLER, S;LACKNER, H;MULLER, W
通讯作者：
MULLER, W