Localization and imaging of gangliosides in mouse brain tissue sections by laserspray ionization inlet[S]
Localization and imaging of gangliosides in mouse brain tissue sections by laserspray ionization inlet[S]
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激光喷雾电离入口对小鼠脑组织切片中神经节苷脂的定位和成像[S]
DOI：
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发表时间：
2012
影响因子：
6.5
通讯作者：
S. Trimpin
中科院分区：
文献类型：
--
作者：
Alicia L. Richards;Christopher B. Lietz;J. Wager;Ken Mackie;S. Trimpin
提出了一种新的用于分析脆性神经节苷脂的离子化方法，该方法没有不希望的碎裂或盐加合。在激光喷雾电离入口（LSII）中，基质/分析物样品在大气压下烧蚀，并且电离通过独立于激光波长或电压的过程在质谱仪入口的离子转移毛细管中发生。LSII的柔软性允许鉴定高达GQ 1的神经节苷脂，唾液酸损失可忽略不计。这对于MS成像领域是重要的，因为不期望的碎片化使得难以准确地绘制组织中脆性神经节苷脂脂质的空间分布。证明了内源性神经节苷脂的原理结构表征，使用多电荷负离子的MSn碎片的LTQ Velos和随后的成像的GD 1神经节苷脂。这是首次报道利用入口电离产生多电荷负离子。我们发现GD 1在小鼠皮层和海马中的水平高于丘脑。在LSII中，激光器在相对于入口的透射几何形状中对准，使用廉价的氮激光器在大约60分钟内获得图像。
A new ionization method for the analysis of fragile gangliosides without undesired fragmentation or salt adduction is presented. In laserspray ionization inlet (LSII), the matrix/analyte sample is ablated at atmospheric pressure, and ionization takes place in the ion transfer capillary of the mass spectrometer inlet by a process that is independent of a laser wavelength or voltage. The softness of LSII allows the identification of gangliosides up to GQ1 with negligible sialic acid loss. This is of importance to the field of MS imaging, as undesired fragmentation has made it difficult to accurately map the spatial distribution of fragile ganglioside lipids in tissue. Proof-of-principle structural characterization of endogenous gangliosides using MSn fragmentation of multiply charged negative ions on a LTQ Velos and subsequent imaging of the GD1 ganglioside is demonstrated. This is the first report of multiply charged negative ions using inlet ionization. We find that GD1 is detected at higher levels in the mouse cortex and hippocampus compared with the thalamus. In LSII with the laser aligned in transmission geometry relative to the inlet, images were obtained in approximately 60 min using an inexpensive nitrogen laser.
DOI：
10.1073/pnas.91.21.9975
发表时间：
1994-10-11
影响因子：
11.1
作者：
YAMANAKA, S;JOHNSON, MD;PROIA, RL
通讯作者：
PROIA, RL
影响因子：
6.8
作者：
Lopez, Pablo H. H.;Schnaar, Ronald L.
通讯作者：
Schnaar, Ronald L.
影响因子：
7.4
作者：
Ivleva, VB;Elkin, YN;Costello, CE
通讯作者：
Costello, CE