Conserved and non-conserved characteristics of porcine glial cell line-derived neurotrophic factor expressed in the testis.
Conserved and non-conserved characteristics of porcine glial cell line-derived neurotrophic factor expressed in the testis.
复制标题
DOI：
10.1038/s41598-018-25924-5
复制
发表时间：
2018-05-16
影响因子：
4.6
通讯作者：
Kubota H
中科院分区：
文献类型：
--
作者：
Kakiuchi K;Taniguchi K;Kubota H
神经胶质细胞系来源的神经营养因子（GDNF）对小鼠、大鼠和家兔精原干细胞（SSCs）的自我更新和增殖至关重要。虽然其他哺乳动物精原细胞增殖的关键外部因素尚未确定，但GDNF是潜在的候选者之一。在这项研究中，我们从新生儿睾丸中分离猪GDNF (pGDNF) cdna，并生成重组pGDNF，以研究其对性腺细胞/未分化精原细胞（包括ssc）的生物学活性。在猪睾丸中，表达了人类和啮齿动物中发现的GDNF pre-(α)pro和pre-(β)pro的长和短形式的转录本。这两个转录本编码相同的成熟蛋白。重组pGDNF在培养中支持小鼠ssc增殖，其干细胞活性通过移植实验得到证实。随后，用pGDNF培养猪性腺细胞/未分化精原细胞；然而，pGDNF不影响它们的增殖。此外，GDNF的表达仅限于血管平滑肌细胞，其同源受体GFRA1在睾丸精原细胞增殖过程中的表达可以忽略不计。这些结果表明，尽管pGDNF保持了与其他哺乳动物的结构相似性，并保留了小鼠ssc自我更新的生物活性，但猪ssc的增殖可能需要GDNF以外的外部因素。
Glial cell line-derived neurotrophic factor (GDNF) is essential for the self-renewal and proliferation of spermatogonial stem cells (SSCs) in mice, rats, and rabbits. Although the key extrinsic factors essential for spermatogonial proliferation in other mammals have not been determined, GDNF is one of the potential candidates. In this study, we isolated porcine GDNF (pGDNF) cDNAs from neonatal testis and generated recombinant pGDNF to investigate its biological activity on gonocytes/undifferentiated spermatogonia, including SSCs. In porcine testis, long and short forms of GDNF transcripts, the counterparts of pre-(α)pro and pre-(β)pro GDNF identified in humans and rodents, were expressed. The two transcripts encode identical mature proteins. Recombinant pGDNF supported proliferation of murine SSCs in culture, and their stem cell activity was confirmed by a transplantation assay. Subsequently, porcine gonocytes/undifferentiated spermatogonia were cultured with pGDNF; however, pGDNF did not affect their proliferation. Furthermore, GDNF expression was localised to the vascular smooth muscle cells, and its cognate receptor GFRA1 expression was negligible during spermatogonial proliferation in the testes. These results indicate that although pGDNF retains structural similarity with those of other mammals and conserves the biological activity on the self-renewal of murine SSCs, porcine SSCs likely require extrinsic factors other than GDNF for their proliferation.
登录
查看更多内容
影响因子：
56.9
作者：
LIN, LFH;DOHERTY, DH;COLLINS, F
通讯作者：
COLLINS, F
DOI：
10.1111/j.1439-0442.1996.tb00442.x
发表时间：
1996-05-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE
影响因子：
--
作者：
Malmgren, L;RodriguezMartinez, H;Einarsson, S
通讯作者：
Einarsson, S
影响因子：
5.3
作者：
Golden, JP;DeMaro, JA;Johnson, EM
通讯作者：
Johnson, EM
影响因子：
5.3
作者：
Lonka-Nevalaita, Liina;Lume, Maria;Saarma, Mart
通讯作者：
Saarma, Mart
影响因子：
3.5
作者：
Grimm, L;Holinski-Feder, E;Ueffing, M
通讯作者：
Ueffing, M