Molecular recognition enables nanosubstrate-mediated delivery of gene-encapsulated nanoparticles with high efficiency.
Molecular recognition enables nanosubstrate-mediated delivery of gene-encapsulated nanoparticles with high efficiency.
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DOI：
10.1021/nn5003024
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发表时间：
2014-05-27
期刊：
影响因子：
17.1
通讯作者：
Tseng, Hsian-Rong
中科院分区：
文献类型：
--
作者：
Peng, Jinliang;Garcia, Mitch Andre;Choi, Jin-sil;Zhao, Libo;Chen, Kuan-Ju;Bernstein, James R.;Peyda, Parham;Hsiao, Yu-Sheng;Liu, Katherine W.;Lin, Wei-Yu;Pyle, April D.;Wang, Hao;Hou, Shuang;Tseng, Hsian-Rong
关键词：
底物介导的基因转移是一种很有前途的方法，由于其独特的能力，预先集中外源基因到指定的基板。然而，许多挑战仍然存在，以使得能够使用相同的基底连续和多轮递送基因，而不在每轮递送中沉积有效载荷和固定细胞。在此，我们介绍了一种基因递送系统，纳米基底介导的递送（NSMD）平台，其基于具有纳米级特征的两个功能组件，包括（1）DNA双链SNPs，用于基因封装的超分子纳米颗粒（SNP）载体，和（2）Ad-SiNWS，金刚烷（Ad）接枝的硅纳米线基底。Ad-SiNWS上的Ad基序和DNA SNPs上的β-环糊精（CD）基序之间的多价分子识别导致DNA SNPs从周围介质动态组装和局部富集到Ad-SiNWS上。随后，一旦细胞沉降在基底上，通过与Ad-SiNWS上的单个纳米线紧密接触，通过细胞膜引入在Ad-SiNWS上富集的DNA SNPs，导致外源基因的高效递送。最重要的是，通过以相等的效率顺序添加相应的DNA SNPs，实现了在沉淀在Ad-SiNWS上的同一批细胞上顺序递送多批外源基因。此外，在体内使用NSMD平台，在皮下移植的Ad-SiNWS上募集的细胞也被负载到SNP中的外源基因有效地转染，验证了该系统在体内的可行性。我们相信这种纳米基质介导的递送平台将为体外和体内基因递送提供上级系统，并且可以进一步用于其他生物分子的包封和递送。
Substrate-mediated gene delivery is a promising method due to its unique ability to preconcentrate exogenous genes onto designated substrates. However, many challenges remain to enable continuous and multiround delivery of the gene using the same substrates without depositing payloads and immobilizing cells in each round of delivery. Herein we introduce a gene delivery system, nanosubstrate-mediated delivery (NSMD) platform, based on two functional components with nanoscale features, including (1) DNA⊂SNPs, supramolecular nanoparticle (SNP) vectors for gene encapsulation, and (2) Ad-SiNWS, adamantane (Ad)-grafted silicon nanowire substrates. The multivalent molecular recognition between the Ad motifs on Ad-SiNWS and the β-cyclodextrin (CD) motifs on DNA⊂SNPs leads to dynamic assembly and local enrichment of DNA⊂SNPs from the surrounding medium onto Ad-SiNWS. Subsequently, once cells settled on the substrate, DNA⊂SNPs enriched on Ad-SiNWS were introduced through the cell membranes by intimate contact with individual nanowires on Ad-SiNWS, resulting in a highly efficient delivery of exogenous genes. Most importantly, sequential delivery of multiple batches of exogenous genes on the same batch cells settled on Ad-SiNWS was realized by sequential additions of the corresponding DNA⊂SNPs with equivalent efficiency. Moreover, using the NSMD platform in vivo, cells recruited on subcutaneously transplanted Ad-SiNWS were also efficiently transfected with exogenous genes loaded into SNPs, validating the in vivo feasibility of this system. We believe that this nanosubstrate-mediated delivery platform will provide a superior system for in vitro and in vivo gene delivery and can be further used for the encapsulation and delivery of other biomolecules.
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影响因子：
16.6
作者：
Gabrielson, Nathan P.;Lu, Hua;Yin, Lichen;Li, Dong;Wang, Fei;Cheng, Jianjun
通讯作者：
Cheng, Jianjun
影响因子：
16.6
作者：
Liu, Yang;Wang, Hao;Kamei, Ken-ichiro;Yan, Ming;Chen, Kuan-Ju;Yuan, Qinghua;Shi, Linqi;Lu, Yunfeng;Tseng, Hsian-Rong
通讯作者：
Tseng, Hsian-Rong
影响因子：
5.1
作者：
通讯作者：
--
影响因子：
14
作者：
Chen, Kuan-Ju;Wolahan, Stephanie M.;Wang, Hao;Hsu, Chao-Hsiung;Chang, Hsing-Wei;Durazo, Armando;Hwang, Lian-Pin;Garcia, Mitch A.;Jiang, Ziyue K.;Wu, Lily;Lin, Yung-Ya;Tseng, Hsian-Rong
通讯作者：
Tseng, Hsian-Rong
DOI：
10.1016/j.ddtec.2005.05.006
发表时间：
2005-01-01
期刊：
Drug discovery today. Technologies
影响因子：
--
作者：
McLennan, Danielle N;Porter, Christopher J H;Charman, Susan A
通讯作者：
Charman, Susan A