A small MRI contrast agent library of gadolinium(III)-encapsulated supramolecular nanoparticles for improved relaxivity and sensitivity.
A small MRI contrast agent library of gadolinium(III)-encapsulated supramolecular nanoparticles for improved relaxivity and sensitivity.
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DOI：
10.1016/j.biomaterials.2010.11.043
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发表时间：
2011-03
期刊：
影响因子：
14
通讯作者：
Tseng, Hsian-Rong
中科院分区：
文献类型：
--
作者：
Chen, Kuan-Ju;Wolahan, Stephanie M.;Wang, Hao;Hsu, Chao-Hsiung;Chang, Hsing-Wei;Durazo, Armando;Hwang, Lian-Pin;Garcia, Mitch A.;Jiang, Ziyue K.;Wu, Lily;Lin, Yung-Ya;Tseng, Hsian-Rong
我们通过包封顺磁性螯合钆（III）引入了一类新的基于纳米颗粒的T1 MRI造影剂（CA），即，Gd 3+·DOTA，通过携带互补分子识别基序的分子构建块的超分子组装，包括金刚烷（Ad）和β-环糊精（CD）。通过系统地改变分子构建块的混合比例，制备了小的Gd 3+·DOTA包封的超分子纳米颗粒（Gd 3+·DOTA-SNPs）库。宽范围的弛豫速率与所得的Gd 3+·DOTA SNP文库相关。因此，Gd 3+·DOTA的最佳合成配方是r1为17.3 s-1 mM-1（约1000）的SNP。4-在高场强下比临床Gd 3+螯合复合物高1倍）。Gd 3+·DOTA SNP的T1加权成像显示出增强的灵敏度，其对比噪声比（C/N比）约为1.5。3.6比游离Gd ~（3+）·DTPA高10倍。将Gd 3+·DOTA双SNP溶液注射到小鼠的足垫中，并且使用MRI来监测基于Gd 3+·DOTA双SNP的CA的动态淋巴引流。我们在T1加权成像中观察到注射Gd 3+·DOTA SNPs后肱淋巴结信号强度增加，但注射Gd 3+·DTPA后没有。MRI结果得到离体ICP-MS分析的支持。这些结果表明，Gd 3+·DOTA双核苷酸单核苷酸多态性不仅表现出增强的弛豫能力和高灵敏度，而且可以作为诊断癌症转移的潜在工具。
We introduce a new category of nanoparticle-based T1 MRI contrast agents (CAs) by encapsulating paramagnetic chelated gadolinium(III), i.e., Gd3+·DOTA, through supramolecular assembly of molecular building blocks that carry complementary molecular recognition motifs, including adamantane (Ad) and β-cyclodextrin (CD). A small library of Gd3+·DOTA-encapsulated supramolecular nanoparticles (Gd3+·DOTA⊂SNPs) was produced by systematically altering the molecular building block mixing ratios. A broad spectrum of relaxation rates was correlated to the resulting Gd3+·DOTA⊂SNP library. Consequently, an optimal synthetic formulation of Gd3+·DOTA⊂SNPs with an r1 of 17.3 s−1mM−1 (ca. 4-fold higher than clinical Gd3+ chelated complexes at high field strengths) was identified. T1-weighted imaging of Gd3+·DOTA⊂SNPs exhibits an enhanced sensitivity with a contrast-to-noise ratio (C/N ratio) ca. 3.6 times greater than that observed for free Gd3+·DTPA. A Gd3+·DOTA⊂SNPs solution was injected into foot pads of mice, and MRI was employed to monitor dynamic lymphatic drainage of the Gd3+·DOTA⊂SNPs-based CA. We observe an increase in signal intensity of the brachial lymph node in T1-weighted imaging after injecting Gd3+·DOTA⊂SNPs but not after injecting Gd3+·DTPA. The MRI results are supported by ICP-MS analysis ex vivo. These results show that Gd3+·DOTA⊂SNPs not only exhibits enhanced relaxivity and high sensitivity but also can serve as a potential tool for diagnosis of cancer metastasis.
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影响因子：
16.6
作者：
Wang, Hao;Wang, Shutao;Su, Helen;Chen, Kuan-Ju;Armijo, Amanda Lee;Lin, Wei-Yu;Wang, Yanju;Sun, Jing;Kamei, Ken-ichiro;Czernin, Johannes;Radu, Caius G.;Tseng, Hsian-Rong
通讯作者：
Tseng, Hsian-Rong
影响因子：
3.5
作者：
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通讯作者：
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影响因子：
15
作者：
Rieter, William J.;Taylor, Kathryn M. L.;Lin, Wenbin
通讯作者：
Lin, Wenbin
影响因子：
4.7
作者：
Rudovsky, Jakub;Botta, Mauro;Aime, Silvio
通讯作者：
Aime, Silvio
影响因子：
3.9
作者：
Vaccaro, Mauro;Accardo, Antonella;Paduano, Luigi
通讯作者：
Paduano, Luigi