Transcriptional Regulations on the Low-Temperature-Induced Floral Transition in an Orchidaceae Species, Dendrobium nobile: An Expressed Sequence Tags Analysis.

Transcriptional Regulations on the Low-Temperature-Induced Floral Transition in an Orchidaceae Species, Dendrobium nobile: An Expressed Sequence Tags Analysis.
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兰科物种金钗石斛低温诱导花转变的转录调控:表达序列标签分析

DOI:
10.1155/2012/757801
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发表时间:
2012
影响因子:
--
通讯作者:
Li HQ
Li HQ
中科院分区:
其他
文献类型:
--
作者:
Liang S;Ye QS;Li RH;Leng JY;Li MR;Wang XJ;Li HQ

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春化诱导开花是许多植物对寒冷环境的适应,但兰科植物开花的遗传基础尚不清楚。在这里,我们报告了15017表达序列标签(EST)的收集从春化腋芽的兰科植物,金钗石斛,组装成9616个独特的基因簇。功能富集分析表明,在40天的春化过程中,与应对压力,特别是在低温的形式,和那些参与蛋白质生物合成和染色质组装的基因显着过量。此外,共识别了59个推定的开花相关基因,包括与温带谷物或拟南芥中春化途径中的已知关键参与者同源的基因,例如谷物VRN 1、FT/VRN 3和拟南芥AGL 19。本研究结果表明,调控春化诱导的花转变的网络在D. nobile、温带谷物和拟南芥。
Vernalization-induced flowering is a cold-relevant adaptation in many species, but little is known about the genetic basis behind in Orchidaceae species. Here, we reported a collection of 15017 expressed sequence tags (ESTs) from the vernalized axillary buds of an Orchidaceae species, Dendrobium nobile, which were assembled for 9616 unique gene clusters. Functional enrichment analysis showed that genes in relation to the responses to stresses, especially in the form of low temperatures, and those involving in protein biosynthesis and chromatin assembly were significantly overrepresented during 40 days of vernalization. Additionally, a total of 59 putative flowering-relevant genes were recognized, including those homologous to known key players in vernalization pathways in temperate cereals or Arabidopsis, such as cereal VRN1, FT/VRN3, and Arabidopsis AGL19. Results from this study suggest that the networks regulating vernalization-induced floral transition are conserved, but just in a part, in D. nobile, temperate cereals, and Arabidopsis.
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影响因子: 7.4
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