Genome organization of epidemic Acinetobacter baumannii strains.
Genome organization of epidemic Acinetobacter baumannii strains.
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DOI：
10.1186/1471-2180-11-224
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发表时间：
2011-10-10
期刊：
影响因子：
4.2
通讯作者：
Zarrilli R
中科院分区：
文献类型：
--
作者：
Di Nocera PP;Rocco F;Giannouli M;Triassi M;Zarrilli R
鲍曼不动杆菌是一种引起医院获得性感染的机会性病原体。据描述，鲍曼不动杆菌在世界范围内的流行是由少数几个基因型群的菌株引起的。在过去的几年里，由被归类为新的基因类型的多重耐药菌株引起的流行病的发生已经被报道。在本研究中，我们比较了三株鲍曼不动杆菌ST2、ST25和ST78的全基因组序列，四株鲍曼不动杆菌的全基因组序列被归类为ST1、ST2和ST77。ST2、ST25和ST78代表了地中海几家医院最常见的流行基因。比较基因组分析表明，鲍曼不动杆菌基因组具有广泛的共性，共鉴定出3068个编码区，它们位于相同的染色体位置。基因组比对还确定了63个DNA区域，大小从4O126kb不等，都被定义为基因组岛，这些区域存在于一些基因组中，但在其他基因组中要么缺失，要么被不同的DNA序列取代。一些岛屿与药物和金属的抗药性有关，另一些岛屿携带编码特定代谢途径中涉及的表面蛋白或酶的基因，还有一些岛屿对应于类似原噬菌体的元件。辅助DNA区域编码12%到19%的分析菌株的潜在基因产物。对鲍曼不动杆菌流行菌株的分析表明，一些岛屿仅限于特定的基因类型。鲍曼不动杆菌基因组组分的定义为快速评估新的临床分离株的基因组结构提供了一个支架。岛屿图谱的改变将有助于鲍曼不动杆菌种群的基因组流行病学研究。
Acinetobacter baumannii is an opportunistic pathogen responsible for hospital-acquired infections. A. baumannii epidemics described world-wide were caused by few genotypic clusters of strains. The occurrence of epidemics caused by multi-drug resistant strains assigned to novel genotypes have been reported over the last few years. In the present study, we compared whole genome sequences of three A. baumannii strains assigned to genotypes ST2, ST25 and ST78, representative of the most frequent genotypes responsible for epidemics in several Mediterranean hospitals, and four complete genome sequences of A. baumannii strains assigned to genotypes ST1, ST2 and ST77. Comparative genome analysis showed extensive synteny and identified 3068 coding regions which are conserved, at the same chromosomal position, in all A. baumannii genomes. Genome alignments also identified 63 DNA regions, ranging in size from 4 o 126 kb, all defined as genomic islands, which were present in some genomes, but were either missing or replaced by non-homologous DNA sequences in others. Some islands are involved in resistance to drugs and metals, others carry genes encoding surface proteins or enzymes involved in specific metabolic pathways, and others correspond to prophage-like elements. Accessory DNA regions encode 12 to 19% of the potential gene products of the analyzed strains. The analysis of a collection of epidemic A. baumannii strains showed that some islands were restricted to specific genotypes. The definition of the genome components of A. baumannii provides a scaffold to rapidly evaluate the genomic organization of novel clinical A. baumannii isolates. Changes in island profiling will be useful in genomic epidemiology of A. baumannii population.
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影响因子：
5.2
作者：
Higgins, Paul G.;Dammhayn, Cathrin;Seifert, Harald
通讯作者：
Seifert, Harald
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影响因子：
4.5
作者：
Eppinger, Mark;Rosovitz, M. J.;Fricke, Wolfgang Florian;Rasko, David A.;Kokorina, Galina;Fayolle, Corinne;Lindler, Luther E.;Carniel, Elisabeth;Ravel, Jacques
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影响因子：
4.8
作者：
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通讯作者：
Filipowicz, W
影响因子：
3.7
作者：
Diancourt L;Passet V;Nemec A;Dijkshoorn L;Brisse S
通讯作者：
Brisse S
影响因子：
3.7
作者：
Darling AE;Mau B;Perna NT
通讯作者：
Perna NT