Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture.
Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture.
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DOI：
10.1016/j.ijpara.2011.10.011
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发表时间：
2012-02
影响因子：
4
通讯作者：
D'Alessandro U
中科院分区：
文献类型：
--
作者：
Borlon C;Russell B;Sriprawat K;Suwanarusk R;Erhart A;Renia L;Nosten F;D'Alessandro U
间日疟原虫体外培养体系的建立对研制新型疫苗、药物和诊断试验具有重要意义。虽然短期培养已经成功建立，但由于需要新鲜的寄生虫分离株，它们在没有直接接触间日疟原虫感染患者的实验室中的重现性受到限制。我们探讨了使用寄生虫分离株和网织红细胞，冷冻保存，进行入侵和启动短期文化的可能性。冻存分离株和网织红细胞的侵袭结果与新鲜样本相似。该方法应易于在流行区以外的实验室重复，并将大大有助于间日疟原虫连续培养的发展。此外，该模型可用于测试候选疫苗以及研究入侵特异性分子机制。
The establishment of a Plasmodium vivax in vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been limited by the need for fresh parasite isolates. We explored the possibility of using parasite isolates and reticulocytes, both cryopreserved, to perform invasion and initiate short-term culture. Invasion results obtained with both cryopreserved isolates and reticulocytes were similar to those obtained with fresh samples. This method should be easily replicated in laboratories outside endemic areas and will substantially contribute to the development of a continuous P. vivax culture. In addition, this model could be used for testing vaccine candidates as well as for studying invasion-specific molecular mechanisms.
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影响因子：
15.8
作者：
Grimberg, Brian T.;Udomsangpetch, Rachanee;King, Christopher L.
通讯作者：
King, Christopher L.
影响因子：
4
作者：
Tasanee, Panichakul;Sattabongkot, Jetsumon;Udomsangpetch, Rachanee
通讯作者：
Udomsangpetch, Rachanee
影响因子：
3
作者：
Sriprawat K;Kaewpongsri S;Suwanarusk R;Leimanis ML;Lek-Uthai U;Phyo AP;Snounou G;Russell B;Renia L;Nosten F
通讯作者：
Nosten F
影响因子：
3
作者：
Sharrock WW;Suwanarusk R;Lek-Uthai U;Edstein MD;Kosaisavee V;Travers T;Jaidee A;Sriprawat K;Price RN;Nosten F;Russell B
通讯作者：
Russell B
DOI：
10.1080/00034983.1983.11811685
发表时间：
1983-01-01
影响因子：
--
作者：
CHRISTOFINIS, GJ;MILLER, H
通讯作者：
MILLER, H