Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood.
Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood.
复制标题
DOI：
10.1186/1475-2875-8-115
复制
发表时间：
2009-06-02
期刊：
影响因子：
3
通讯作者：
Nosten F
中科院分区：
文献类型：
--
作者：
Sriprawat K;Kaewpongsri S;Suwanarusk R;Leimanis ML;Lek-Uthai U;Phyo AP;Snounou G;Russell B;Renia L;Nosten F
间日疟原虫的研究仅限于从受感染的人或灵长类动物收集的样本，因为这种寄生虫不能在体外培养中维持。间日疟原虫分离株被宿主白细胞和血小板污染对一系列离体和分子研究是有害的。易于生产的CF 11纤维素过滤器最近为我们提供了一种廉价的方法来去除白细胞和血小板。这与先前报道的CF 11对感染红细胞（IRBC）的保留水平高得不可接受形成对比。本研究的目的是比较CF 11纤维素过滤器和市售Plasmodipur过滤器去除白细胞和血小板的能力，并研究CF 11纤维素过滤器对间日疟原虫IRBC的截留。使用来自5名健康供体的血液样本对6种白细胞去除方法进行的并行比较显示，CF 11过滤将平均初始白细胞计数从9.4 × 103/μl [95%CI 5.2-13.5]降至0.01 × 103 [95%CI 0.01-0.03]。CF 11在去除中性粒细胞方面特别有效。CF 11治疗还使初始血小板计数从211.6 × 103/μl [95%CI 107.5-315.7]降至0.8 × 103/μl [95%CI -0.7-2.2]。对CF 11过滤前后的30份间日疟原虫血液样本进行分析，结果显示寄生虫血症仅有轻微损失（≤初始计数的7.1%）。未观察到间日疟原虫IRBC的阶段特异性保留。CF 11过滤是从田间分离株中生产无白细胞和无血小板间日疟原虫感染红细胞的最具成本和时间效益的方法。
Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo and molecular investigations. Easy-to-produce CF11 cellulose filters have recently provided us with an inexpensive method for the removal of leukocytes and platelets. This contrasted with previous reports of unacceptably high levels of infected red blood cell (IRBC) retention by CF11. The aims of this study were to compare the ability of CF11 cellulose filters and the commercial filter Plasmodipur at removing leukocyte and platelet, and to investigate the retention of P. vivax IRBCs by CF11 cellulose filtration. Side-by-side comparison of six leukocyte removal methods using blood samples from five healthy donor showed that CF11 filtration reduced the mean initial leukocyte counts from 9.4 × 103 per μl [95%CI 5.2–13.5] to 0.01 × 103 [95%CI 0.01–0.03]. The CF11 was particularly effective at removing neutrophils. CF11 treatment also reduced initial platelet counts from 211.6 × 103 per μl [95%CI 107.5–315.7] to 0.8 × 103 per μl [95%CI -0.7–2.2]. Analysis of 30 P. vivax blood samples before and after CF11 filtration showed only a minor loss in parasitaemia (≤ 7.1% of initial counts). Stage specific retention of P. vivax IRBCs was not observed. CF11 filtration is the most cost and time efficient method for the production of leukocyte- and platelet-free P. vivax-infected erythrocytes from field isolates.
登录
查看更多内容
影响因子：
64.8
作者：
Carlton, Jane M.;Adams, John H.;Silva, Joana C.;Bidwell, Shelby L.;Lorenzi, Hernan;Caler, Elisabet;Crabtree, Jonathan;Angiuoli, Samuel V.;Merino, Emilio F.;Amedeo, Paolo;Cheng, Qin;Coulson, Richard M. R.;Crabb, Brendan S.;del Portillo, Hernando A.;Essien, Kobby;Feldblyum, Tamara V.;Fernandez-Becerra, Carmen;Gilson, Paul R.;Gueye, Amy H.;Guo, Xiang;Kang'a, Simon;Kooij, Taco W. A.;Korsinczky, Michael;Meyer, Esmeralda V. -S.;Nene, Vish;Paulsen, Ian;White, Owen;Ralph, Stuart A.;Ren, Qinghu;Sargeant, Tobias J.;Salzberg, Steven L.;Stoeckert, Christian J.;Sullivan, Steven A.;Yamamoto, Marcio M.;Hoffman, Stephen L.;Wortman, Jennifer R.;Gardner, Malcolm J.;Galinski, Mary R.;Barnwell, John W.;Fraser-Liggett, Claire M.
通讯作者：
Fraser-Liggett, Claire M.
影响因子：
3.7
作者：
Suwanarusk R;Russell B;Chavchich M;Chalfein F;Kenangalem E;Kosaisavee V;Prasetyorini B;Piera KA;Barends M;Brockman A;Lek-Uthai U;Anstey NM;Tjitra E;Nosten F;Cheng Q;Price RN
通讯作者：
Price RN
DOI：
10.1080/00034983.1973.11686885
发表时间：
1973-01-01
影响因子：
--
作者：
RICHARDS, WH;WILLIAMS, SG
通讯作者：
WILLIAMS, SG
影响因子：
--
作者：
Le, Minh-Tam;Bretschneider, Timo R.;Preiser, Peter R.
通讯作者：
Preiser, Peter R.
DOI：
10.1073/pnas.0807404105
发表时间：
2008-10-21
影响因子：
11.1
作者：
Bozdech, Zbynek;Mok, Sachel;Preiser, Peter R.
通讯作者：
Preiser, Peter R.