Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates.
Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates.
复制标题
鱼类的缺氧耐受性取决于脂质和碳水化合物之间的分解代谢偏好。
DOI：
10.24272/j.issn.2095-8137.2023.098
复制
发表时间：
2023-09-18
影响因子：
4.9
通讯作者：
中科院分区：
文献类型：
--
作者：
低氧是水生生物常见的环境应激因子，不同鱼种的环境应激因子不同。然而，鱼类耐受低氧能力的潜在机制还不是很清楚。在这里，我们表明，不同物种的低氧反应受到脂肪分解代谢和对脂肪或碳水化合物能量来源的偏好的影响。罗非鱼体内通过过氧化物酶体增殖物激活受体α激活生化脂质分解代谢或增加线粒体脂肪氧化，通过增加耗氧量和氧化损伤，减少作为能量来源的碳水化合物分解代谢，从而降低对急性缺氧的耐受性。相反，通过抑制脂质进入罗非鱼线粒体或单独敲除斑马鱼脂质分解代谢的三个关键基因来抑制脂质分解代谢，通过减少耗氧量和氧化损伤，促进碳水化合物分解代谢，提高了对急性缺氧的耐受性。然而，在脂肪甘油三酯脂肪酶(ATGL)突变的斑马鱼中，抑制厌氧糖酵解消除了脂肪分解代谢的抑制，促进了低氧耐受。在14种不同营养水平和分类地位的鱼类中，优先利用脂肪作为能量的鱼类比优先利用碳水化合物的鱼类对急性缺氧的耐受性更强。我们的研究表明，鱼类对低氧的耐受依赖于对脂类或碳水化合物的分解代谢偏好，这种偏好可以通过调节脂类分解代谢来改变。
Hypoxia is a common environmental stress factor in aquatic organisms, which varies among fish species. However, the mechanisms underlying the ability of fish species to tolerate hypoxia are not well known. Here, we showed that hypoxia response in different fish species was affected by lipid catabolism and preference for lipid or carbohydrate energy sources. Activation of biochemical lipid catabolism through peroxisome proliferator-activated receptor alpha (Pparα) or increasing mitochondrial fat oxidation in tilapia decreased tolerance to acute hypoxia by increasing oxygen consumption and oxidative damage and reducing carbohydrate catabolism as an energy source. Conversely, lipid catabolism inhibition by suppressing entry of lipids into mitochondria in tilapia or individually knocking out three key genes of lipid catabolism in zebrafish increased tolerance to acute hypoxia by decreasing oxygen consumption and oxidative damage and promoting carbohydrate catabolism. However, anaerobic glycolysis suppression eliminated lipid catabolism inhibition-promoted hypoxia tolerance in adipose triglyceride lipase (atgl) mutant zebrafish. Using 14 fish species with different trophic levels and taxonomic status, the fish preferentially using lipids for energy were more intolerant to acute hypoxia than those preferentially using carbohydrates. Our study shows that hypoxia tolerance in fish depends on catabolic preference for lipids or carbohydrates, which can be modified by regulating lipid catabolism.
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DOI：
10.1016/s0360-3016(02)02848-1
发表时间：
2002-08-01
影响因子：
7
作者：
Koukourakis, MI;Giatromanolaki, A;Harris, AL
通讯作者：
Harris, AL
影响因子：
2.9
作者：
Ma, Qiang;Hu, Chun-Ting;Du, Zhen-Yu
通讯作者：
Du, Zhen-Yu
影响因子：
3.7
作者：
Almeida, David;Merino-Aguirre, Raquel;Copp, Gordon H.
通讯作者：
Copp, Gordon H.
过氧化物酶体增殖物激活受体α-b缺乏诱导斑马鱼营养代谢重编程
影响因子：
5.5
作者：
Li, Ling-Yu;Lv, Hong-Bo;Du, Zhen-Yu
通讯作者：
Du, Zhen-Yu
影响因子：
2
作者：
Chagas, E. C.;Val, A. L.
通讯作者：
Val, A. L.