Motion-Based Immunological Detection of Zika Virus Using Pt-Nanomotors and a Cellphone.
Motion-Based Immunological Detection of Zika Virus Using Pt-Nanomotors and a Cellphone.
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DOI：
10.1021/acsnano.8b01515
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发表时间：
2018-06-26
期刊：
影响因子：
17.1
通讯作者：
Shafiee H
中科院分区：
文献类型：
--
作者：
Draz MS;Lakshminaraasimulu NK;Krishnakumar S;Battalapalli D;Vasan A;Kanakasabapathy MK;Sreeram A;Kallakuri S;Thirumalaraju P;Li Y;Hua S;Yu XG;Kuritzkes DR;Shafiee H
寨卡病毒（ZIKV）感染是对人类的一种新出现的大流行威胁，对新生儿可能致命。数字卫生系统和纳米颗粒的进步可以促进开发敏感和便携式检测技术，以便及时管理新出现的病毒感染。本文报道了一种基于纳米马达的头动手机（NBC）免疫检测系统。病毒在测试样品中的存在导致铂（Pt）纳米马达在珠子表面的积累，导致它们在H2O2溶液中运动。然后检测病毒浓度与珠粒运动变化的相关性。该系统能在低至1粒/μL的样品中检测到寨卡病毒。NBC系统允许在密切相关的登革热病毒和其他嗜神经病毒（如1型单纯疱疹病毒和人巨细胞病毒）存在的情况下，高度特异性地检测寨卡病毒。在发达国家和发展中国家，NBC平台技术有潜力用于开发用于病原体检测和疾病管理的即时诊断。
Zika virus (ZIKV) infection is an emerging pandemic threat to humans that can be fatal in newborns. Advances in digital health systems and nanoparticles can facilitate the development of sensitive and portable detection technologies for timely management of emerging viral infections. Here we report a nanomotor-based bead-motion cellphone (NBC) system for the immunological detection of ZIKV. The presence of virus in a testing sample results in the accumulation of platinum (Pt)-nanomotors on the surface of beads, causing their motion in H2O2 solution. Then the virus concentration is detected in correlation with the change in beads motion. The developed NBC system was capable of detecting ZIKV in samples with virus concentrations as low as 1 particle/μL. The NBC system allowed a highly specific detection of ZIKV in the presence of the closely related dengue virus and other neurotropic viruses, such as herpes simplex virus type 1 and human cytomegalovirus. The NBC platform technology has the potential to be used in the development of point-of-care diagnostics for pathogen detection and disease management in developed and developing countries.
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