The role of calretinin-expressing granule cells in olfactory bulb functions and odor behavior.
The role of calretinin-expressing granule cells in olfactory bulb functions and odor behavior.
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DOI：
10.1038/s41598-018-27692-8
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发表时间：
2018-06-20
影响因子：
4.6
通讯作者：
Saghatelyan A
中科院分区：
文献类型：
--
作者：
Hardy D;Malvaut S;Breton-Provencher V;Saghatelyan A
成年小鼠嗅球持续供应新的神经元，主要分化为颗粒细胞（GC）。不同亚型的成人出生的GC已被确定，但他们的成熟配置文件和他们的作用，延髓网络功能和气味行为仍然难以捉摸。同样不清楚的是，在出生后早期（早期出生）或在成年期（成年出生）出生的GC的相同亚群是否在其形态功能特性上有所不同。在这里，我们发现，成人出生的钙视黄蛋白表达（CR+）和非表达（CR-）GC，以及早期出生的CR+ GC，显示不同的抑制性输入，但难以区分的兴奋性输入和相似的形态特征。抑制性突触后电流的频率在早期出生和成年出生的CR+ GC中低于成年出生的CR−神经元。CR+ GC上桥蛋白+斑点密度降低证实了这些发现。根据立即早期基因表达分析，CR+ GC在基于气味辨别的嗅觉任务后显示出更高水平的激活。CR+ GC的药物遗传学抑制降低了小鼠辨别复杂气味混合物的能力。总之，我们的研究结果表明，不同的抑制性输入接收的成年出生的CR+和CR− GC，早期和成年出生的CR+神经元具有相似的形态功能特性，CR+ GC参与复杂的气味辨别任务。
The adult mouse olfactory bulb is continuously supplied with new neurons that mostly differentiate into granule cells (GCs). Different subtypes of adult-born GCs have been identified, but their maturational profiles and their roles in bulbar network functioning and odor behavior remain elusive. It is also not known whether the same subpopulations of GCs born during early postnatal life (early-born) or during adulthood (adult-born) differ in their morpho-functional properties. Here, we show that adult-born calretinin-expressing (CR+) and non-expressing (CR−) GCs, as well as early-born CR+ GCs, display distinct inhibitory inputs but indistinguishable excitatory inputs and similar morphological characteristics. The frequencies of inhibitory post-synaptic currents were lower in early-born and adult-born CR+ GCs than in adult-born CR− neurons. These findings were corroborated by the reduced density of gephyrin+ puncta on CR+ GCs. CR+ GCs displayed a higher level of activation following olfactory tasks based on odor discrimination, as determined by an immediate early gene expression analysis. Pharmacogenetic inhibition of CR+ GCs diminished the ability of the mice to discriminate complex odor mixtures. Altogether, our results indicate that distinct inhibitory inputs are received by adult-born CR+ and CR− GCs, that early- and adult-born CR+ neurons have similar morpho-functional properties, and that CR+ GCs are involved in complex odor discrimination tasks.
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DOI：
10.1073/pnas.90.8.3329
发表时间：
1993-04-15
影响因子：
11.1
作者：
GUTHRIE, KM;ANDERSON, AJ;GALL, C
通讯作者：
GALL, C
影响因子：
25
作者：
Imayoshi, Itaru;Sakamoto, Masayuki;Kageyama, Ryoichiro
通讯作者：
Kageyama, Ryoichiro
影响因子：
4.2
作者：
Jakobsson, J;Ericson, C;Lundberg, C
通讯作者：
Lundberg, C
影响因子：
5.3
作者：
Lemasson, M;Saghatelyan, A;Lledo, PM
通讯作者：
Lledo, PM
影响因子：
25
作者：
Alonso, Mariana;Lepousez, Gabriel;Lledo, Pierre-Marie
通讯作者：
Lledo, Pierre-Marie