SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes.
SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes.
复制标题
SabR 通过调节 sanG（产色链霉菌中的一种途径特异性调节基因）的转录水平来增强尼可霉素的产量。
DOI：
10.1186/1471-2180-11-164
复制
发表时间：
2011-07-20
期刊：
影响因子：
4.2
通讯作者：
Tan H
中科院分区：
文献类型：
--
作者：
Pan Y;Wang L;He X;Tian Y;Liu G;Tan H
sabR是一个多效性调控基因，已被证明对尼克霉素的生物合成具有正调控作用，而对反色链霉菌的产孢具有负调控作用。在这项研究中，我们研究了SabR在链霉菌反色基因中调节尼克霉素生产的机制。sabR的转录起始点由高分辨率S1核酸酶作图确定，定位于sabR潜在翻译起始密码子（GTG）上游37bp的核苷酸T位置。sabR的破坏增强了其自身的转录，但延缓了nikkomycin的产生。sabR过表达增强了链霉菌反色基因中尼克霉素的生物合成。EMSA分析表明，SabR结合到sanG的上游区域，但没有结合到其编码基因（SabR）的上游区域sanF和sanN与sanO之间的基因间区。DNase 1足迹分析显示，sanG上游的sabr结合位点为5'-CTTTAAGTCACCTGGCTCATTCGCGTTCGCCCAGCT-3'，命名为SARE。SARE的缺失导致尼克霉素产生的延迟，这与sabR破坏突变体相似。这些结果表明SabR作为增强子通过与sanG启动子区相互作用来调节尼克霉素的生物合成，并扩大了我们对尼克霉素生物合成调控级联的理解。
sabR is a pleiotropic regulatory gene which has been shown to positively regulate the nikkomycin biosynthesis and negatively affect the sporulation of Streptomyces ansochromogenes. In this study, we investigate the mechanism of SabR on modulating nikkomycin production in Streptomyces ansochromogenes. The transcription start point of sabR was determined by high-resolution S1 nuclease mapping and localized at the nucleotide T at position 37 bp upstream of the potential sabR translation start codon (GTG). Disruption of sabR enhanced its own transcription, but retarded the nikkomycin production. Over-expression of sabR enhanced nikkomycin biosynthesis in Streptomyces ansochromogenes. EMSA analysis showed that SabR bound to the upstream region of sanG, but it did not bind to the upstream region of its encoding gene (sabR), sanF and the intergenic region between sanN and sanO. DNase 1 footprinting assays showed that the SabR-binding site upstream of sanG was 5'-CTTTAAGTCACCTGGCTCATTCGCGTTCGCCCAGCT-3' which was designated as SARE. Deletion of SARE resulted in the delay of nikkomycin production that was similar to that of sabR disruption mutant. These results indicated that SabR modulated nikkomycin biosynthesis as an enhancer via interaction with the promoter region of sanG, and expanded our understanding about regulatory cascade in nikkomycin biosynthesis.
登录
查看更多内容
影响因子：
5.1
作者：
Nishida, Hiromi;Ohnishi, Yasuo;Horinouchi, Sueharu
通讯作者：
Horinouchi, Sueharu
DOI：
10.1007/s10295-003-0063-z
发表时间：
2003-08-01
影响因子：
3.4
作者：
Horinouchi, S
通讯作者：
Horinouchi, S
影响因子：
3.6
作者：
Li, Rui;Liu, Gang;Tan, Huarong
通讯作者：
Tan, Huarong
影响因子：
2.1
作者：
Ishikawa, J;Hotta, K
通讯作者：
Hotta, K
影响因子：
5
作者：
Engel, P;Scharfenstein, LL;Cary, JW
通讯作者：
Cary, JW