A versatile valving toolkit for automating fluidic operations in paper microfluidic devices.
A versatile valving toolkit for automating fluidic operations in paper microfluidic devices.
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DOI：
10.1039/c4lc01155d
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发表时间：
2015-03-21
期刊：
影响因子：
6.1
通讯作者：
Yager P
中科院分区：
文献类型：
--
作者：
Toley BJ;Wang JA;Gupta M;Buser JR;Lafleur LK;Lutz BR;Fu E;Yager P
未能利用阀门和自动化技术限制了流体操作的复杂性，可以在纸微流体装置中执行。我们开发了一个纸微流体阀的工具包，以及使用可移动纸条和流体触发膨胀元件的自动阀门驱动方法。据我们所知，这是该阀控策略在纸微流体中的首次功能演示。在设备上引入流体后，阀门可以在a)一段时间后或b)经过一定体积的流体后自动启动。通过改变定时灯芯的长度，可以以超过8.5%的精度调整阀门的执行时间，并且我们提供定时的阀上、阀下和导流（通道切换）阀。执行器需要~30 μl的流体来驱动，从一种状态切换到另一种状态所需的时间范围从短~5 s到长~50s。对于体积计驱动，可以调整计量垫的大小来调整驱动体积，我们提出了两种方法-两种方法都可以实现大于9%的精度。最后，我们演示了在一种设备中使用这些阀门进行多步骤检测，以检测疟疾蛋白PfHRP2。虽然比没有移动部件的设备稍微复杂一些，但这个阀门和自动化工具包大大扩展了纸微流体设备的功能。该工具包的组件可用于在纸质设备上进行任意复杂的多步骤流体操作，如疟疾检测设备所示。
Failure to utilize valving and automation techniques has restricted the complexity of fluidic operations that can be performed in paper microfluidic devices. We developed a toolkit of paper microfluidic valves and methods for automatic valve actuation using movable paper strips and fluid-triggered expanding elements. To the best of our knowledge, this is the first functional demonstration of this valving strategy in paper microfluidics. After introduction of fluids on devices, valves can actuate automatically a) after a certain period of time, or b) after the passage of a certain volume of fluid. Timing of valve actuation can be tuned with greater than 8.5% accuracy by changing lengths of timing wicks, and we present timed on-valves, off-valves, and diversion (channel-switching) valves. The actuators require ~30 μl fluid to actuate and the time required to switch from one state to another ranges from ~5 s for short to ~50s for longer wicks. For volume-metered actuation, the size of a metering pad can be adjusted to tune actuation volume, and we present two methods – both methods can achieve greater than 9% accuracy. Finally, we demonstrate the use of these valves in a device that conducts a multi-step assay for the detection of the malaria protein PfHRP2. Although slightly more complex than devices that do not have moving parts, this valving and automation toolkit considerably expands the capabilities of paper microfluidic devices. Components of this toolkit can be used to conduct arbitrarily complex, multi-step fluidic operations on paper-based devices, as demonstrated in the malaria assay device.
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影响因子：
6.2
作者：
Bhakta SA;Borba R;Taba M Jr;Garcia CD;Carrilho E
通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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