Determination of nitrite in saliva using microfluidic paper-based analytical devices.
Determination of nitrite in saliva using microfluidic paper-based analytical devices.
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DOI：
10.1016/j.aca.2013.11.044
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发表时间：
2014-01-27
影响因子：
6.2
通讯作者：
Carrilho E
中科院分区：
文献类型：
--
作者：
Bhakta SA;Borba R;Taba M Jr;Garcia CD;Carrilho E
即时护理平台可以提供快速响应，降低治疗的总成本，允许在有或没有受过训练的专家的情况下进行家庭确定，并提高治疗的成功率。对于微流体纸基分析设备（μPAD）来说尤其如此，它可以开发出高效和多功能的分析工具，并应用于各种生物医学领域。这项工作的目的是开发μ PAD来识别和量化唾液中亚硝酸盐的水平，亚硝酸盐已被提议作为牙周炎的潜在标志物。该装置通过蜡印刷制造，并允许通过基于Griess反应的修改版本的比色反应来检测亚硝酸盐。所提出的修改，沿着纸基平台的实现，解决了许多常见的缺点（显色，稳定性等）。与Griess反应相关，并得到与申报器械的设计、表征和应用相关的结果的支持。在优化的条件下，所提出的装置能够在10至1000 μmol L−1范围内测定亚硝酸盐，检测限为10 μmol L−1，灵敏度为47.5 Au [log（μmol L−1）]−1。为了证明该技术在医疗保健行业的潜在影响，将该设备应用于一系列真实的样本的分析，涵盖了相关的临床范围。
Point-of-care platforms can provide fast responses, decrease the overall cost of the treatment, allow for in-home determinations with or without a trained specialist, and improve the success of the treatment. This is especially true for microfluidic paper-based analytical devices (μPAD), which can enable the development of highly efficient and versatile analytical tools with applications in a variety of biomedical fields. The objective of this work was the development of μPADs to identify and quantify levels of nitrite in saliva, which has been proposed as a potential marker of periodontitis. The devices were fabricated by wax printing and allowed the detection of nitrite by a colorimetric reaction based on a modified version of the Griess reaction. The presented modifications, along with the implementation of a paper-based platform, address many of the common drawbacks (color development, stability, etc.) associated with the Griess reaction and are supported by results related to the design, characterization, and application of the proposed devices. Under the optimized conditions, the proposed devices enable the determination of nitrite in the 10 to 1000 μmol L−1 range with a limit of detection of 10 μmol L−1 and a sensitivity of 47.5 AU [log (μmol L−1)]−1. In order to demonstrate the potential impact of this technology in the healthcare industry, the devices were applied to the analysis of a series of real samples, covering the relevant clinical range.
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一氧化氮作为牙周炎中的炎症标志物的估计。
影响因子：
--
作者：
Menaka, K B;Ramesh, Amitha;Thomas, Biju;Kumari, N Suchetha
通讯作者：
Kumari, N Suchetha
影响因子：
6.1
作者：
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影响因子：
2.1
作者：
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影响因子：
3.4
作者：
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通讯作者：
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影响因子：
6.7
作者：
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通讯作者：
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