Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability.
Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability.
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DOI：
10.1083/jcb.200705145
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发表时间：
2007-12-17
期刊：
影响因子：
--
通讯作者：
Lupashin V
中科院分区：
文献类型：
--
作者：
Shestakova A;Suvorova E;Pavliv O;Khaidakova G;Lupashin V
系留因子介导转运囊泡与靶膜的初始相互作用。可溶性N-乙基马来酰亚胺敏感的融合蛋白附着蛋白受体(SNARE)使随后的对接和膜融合成为可能。我们证明了囊泡系留保守的寡聚体高尔基体(COG)复合体与高尔基体内的陷阱分子共定位和免疫共沉淀。在酵母细胞中，COG复合体优先与含有酵母高尔基靶标(T)-SNARE Sed5p的SNARE复合体相互作用。在哺乳动物细胞中，hCog4p和hCog6p与Sed5p的哺乳动物同源物Synaxin5a相互作用。此外，荧光共振能量转移揭示了Synaxin5a与COG复合体之间的体内相互作用。哺乳动物COG复合体的敲除降低了高尔基体圈套的流动性，产生了自由突触蛋白5的积累，并降低了高尔基体内圈套复合体的稳态水平。最后，hCog4p N-末端Synaxin5a结合域的过表达破坏了高尔基体内SNARE复合体的稳定性，破坏了高尔基体。这些数据表明，COG复合体通过与t-SNARE Synaxin5a/Sed5p结合并增强高尔基体内SNARE复合体的稳定性，在酵母和哺乳动物细胞中类似地协调囊泡的运输。
Tethering factors mediate initial interaction of transport vesicles with target membranes. Soluble N-ethylmaleimide–sensitive fusion protein attachment protein receptors (SNAREs) enable consequent docking and membrane fusion. We demonstrate that the vesicle tether conserved oligomeric Golgi (COG) complex colocalizes and coimmunoprecipitates with intra-Golgi SNARE molecules. In yeast cells, the COG complex preferentially interacts with the SNARE complexes containing yeast Golgi target (t)-SNARE Sed5p. In mammalian cells, hCog4p and hCog6p interact with Syntaxin5a, the mammalian homologue of Sed5p. Moreover, fluorescence resonance energy transfer reveals an in vivo interaction between Syntaxin5a and the COG complex. Knockdown of the mammalian COG complex decreases Golgi SNARE mobility, produces an accumulation of free Syntaxin5, and decreases the steady-state levels of the intra-Golgi SNARE complex. Finally, overexpression of the hCog4p N-terminal Syntaxin5a-binding domain destabilizes intra-Golgi SNARE complexes, disrupting the Golgi. These data suggest that the COG complex orchestrates vesicular trafficking similarly in yeast and mammalian cells by binding to the t-SNARE Syntaxin5a/Sed5p and enhancing the stability of intra-Golgi SNARE complexes.
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影响因子：
64.5
作者：
NOVICK, P;FIELD, C;SCHEKMAN, R
通讯作者：
SCHEKMAN, R
影响因子：
3.3
作者：
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影响因子：
4.5
作者：
Fridmann-Sirkis, Y;Kent, HM;Pelham, HRB
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Pelham, HRB
影响因子：
4.5
作者：
Shestakova, A;Zolov, S;Lupashin, V
通讯作者：
Lupashin, V
影响因子：
3.3
作者：
Jones, S;Newman, C;Segev, N
通讯作者：
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