Expression characteristics of the plasmid-borne mcr-1 colistin resistance gene.
Expression characteristics of the plasmid-borne mcr-1 colistin resistance gene.
复制标题
质粒携带的 mcr-1 粘菌素抗性基因的表达特征。
DOI：
10.18632/oncotarget.22538
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发表时间：
2017-12-08
期刊：
影响因子：
--
通讯作者：
Du H
中科院分区：
文献类型：
--
作者：
Zhang H;Miao M;Yan J;Wang M;Tang YW;Kreiswirth BN;Zhang X;Chen L;Du H
The plasmid-encoded colistin resistance gene (mcr-1) has recently been reported in various Gram-negative species. However, the expression profile of mcr-1 remains unknown. Here, we investigated the expression of mcr-1 in various plasmids and bacteria. The mcr-1 expression levels in pMCR1_IncX4 varied from 1.81 × 10–5 to 1.05 × 10–4 (pmol per μg total RNA) in the two K. pneumoniae strains SZ03 and SZ04 (ST25) and the two E. coli strains SZ01 and CDA6 (ST2448 and ST167, respectively). The mcr-1 expression levels of pMCR1_IncI2 in E. coli SZ02 (ST2085) and E. coli BJ13 (ST457) were 5.27 × 10–5 and 2.58 × 10–5, respectively. In addition, the expression of chromosomal mcr-1 in ST156 E. coli BJ10 was 5.49×10–5. Interestingly, after 4μg/ml colistin treatment, mcr-1 in pMCR1_IncX4 increased 2- and 4-fold at 20 and 120 mins, respectively, in all pMCR1_IncX4-harboring strains, except for ST2448 E. coli, which had a lower expression after 20 mins that restored to baseline levels after 120 mins. In contrast, mcr-1 expression of pMCR1_IncI2 in the two E. coli strains (SZ02, BJ13) and the chromosomal mcr-1 in E. coli (BJ10) remained at baseline levels after 20 and 120 mins. In the same genetic background host strain E. coli E600, mcr-1 expression of pMCR1_IncX4 and pMCR1_IncI2 were similar and were decreased after colistin treatment for 20 min. However, mcr-1 in pMCR1_IncX4 was up-regulated after colistin treatment for 120 min, while mcr-1 in pMCR1_IncI2 was down-regulated compared to the untreated control. Our results suggested that mcr-1 has distinct expression profiles on different plasmids, bacterial hosts, and after antibiotic treatment.
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影响因子：
4.9
作者：
Poirel, Laurent;Kieffer, Nicolas;Nordmann, Patrice
通讯作者：
Nordmann, Patrice
影响因子：
5.2
作者：
Schwarz, Stefan;Johnson, Alan P.
通讯作者：
Johnson, Alan P.
影响因子：
56.3
作者：
Liu, Yi-Yun;Wang, Yang;Shen, Jianzhong
通讯作者：
Shen, Jianzhong
RpoE 通过调节伤寒沙门氏菌中的 SPI-1 和 SPI-2 促进侵袭和细胞内存活。
影响因子：
3.1
作者：
Zhang, Haifang;Jia, Yanwei;Du, Hong
通讯作者：
Du, Hong
DOI：
10.1016/s1473-3099(13)70190-7
发表时间：
2013-09
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Munoz-Price LS;Poirel L;Bonomo RA;Schwaber MJ;Daikos GL;Cormican M;Cornaglia G;Garau J;Gniadkowski M;Hayden MK;Kumarasamy K;Livermore DM;Maya JJ;Nordmann P;Patel JB;Paterson DL;Pitout J;Villegas MV;Wang H;Woodford N;Quinn JP
通讯作者：
Quinn JP