The mitochondrial genome of Muga silkworm (Antheraea assamensis) and its comparative analysis with other lepidopteran insects.
The mitochondrial genome of Muga silkworm (Antheraea assamensis) and its comparative analysis with other lepidopteran insects.
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DOI：
10.1371/journal.pone.0188077
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Bora U
中科院分区：
文献类型：
--
作者：
Singh D;Kabiraj D;Sharma P;Chetia H;Mosahari PV;Neog K;Bora U
Muga是印度阿萨姆邦和梅加拉亚邦特有的一种重要的经济蚕蛾，是已知最坚固的商业丝绸的生产商。然而，在分子水平上理解生物体的基因组和蛋白质组数据是稀缺的。我们的研究是利用下一代测序技术对烟夜蛾线粒体全基因组(有丝分裂基因组)进行解码，并将其与其他鳞翅目有丝分裂基因组进行比较。Asamens的有丝分裂组是一个富含AT的环状分子，全长15,272bp(A+T含量~80.2%)。它包含37个基因，包括13个蛋白质编码基因(PCGs)、22个tRNA和2个rRNA基因以及一个328个核苷酸的控制区。其典型的tRNAMet-tRNAIle-tRNAGln排列不同于祖先昆虫(tRNAIle-tRNAGln-tRNAMet)。发现两个PCG COX1和COX2的起始密码子分别为CGA和GTG，这在一些鳞翅目昆虫中已有报道。有趣的是，nad4l基因在种内的颠换突变高于种间。所有的PCG都是在强纯化选择下进化的，其中ATP8基因的进化速率最高，而Cox1基因的进化速率最低。我们观察到tRNAs典型的三叶状二级结构，但在tRNASer1和tRNatyr的情况下例外，其中没有稳定的Dhu和TΨC环。大量的错配(35个)分布在19个tRNA结构中。有丝分裂组的控制区含有一个非典型的6个碱基(CTTAGA/G)缺失，缺乏串联重复序列。该属的系统发育地位与传统的土豆科分类一致。完整的有丝分裂基因组注释可在GenBank(登录号：KU379695)。据我们所知，这是关于Asamens完整有丝分裂组的第一次报道。
Muga (Antheraea assamensis) is an economically important silkmoth endemic to the states of Assam and Meghalaya in India and is the producer of the strongest known commercial silk. However, there is a scarcity of genomic and proteomic data for understanding the organism at a molecular level. Our present study is on decoding the complete mitochondrial genome (mitogenome) of A. assamensis using next generation sequencing technology and comparing it with other available lepidopteran mitogenomes. Mitogenome of A. assamensis is an AT rich circular molecule of 15,272 bp (A+T content ~80.2%). It contains 37 genes comprising of 13 protein coding genes (PCGs), 22 tRNA and 2 rRNA genes along with a 328 bp long control region. Its typical tRNAMet-tRNAIle-tRNAGln arrangement differed from ancestral insects (tRNAIle-tRNAGln-tRNAMet). Two PCGs cox1 and cox2 were found to have CGA and GTG as start codons, respectively as reported in some lepidopterans. Interestingly, nad4l gene showed higher transversion mutations at intra-species than inter-species level. All PCGs evolved under strong purifying selection with highest evolutionary rates observed for atp8 gene while lowest for cox1 gene. We observed the typical clover-leaf shaped secondary structures of tRNAs with a few exceptions in case of tRNASer1 and tRNATyr where stable DHU and TΨC loop were absent. A significant number of mismatches (35) were found to spread over 19 tRNA structures. The control region of mitogenome contained a six bp (CTTAGA/G) deletion atypical of other Antheraea species and lacked tandem repeats. Phylogenetic position of A. assamensis was consistent with the traditional taxonomic classification of Saturniidae. The complete annotated mitogenome is available in GenBank (Accession No. KU379695). To the best of our knowledge, this is the first report on complete mitogenome of A. assamensis.
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