GISH, AFLP and PCR-RFLP analysis of an intergeneric somatic hybrid combining Goutou sour orange and Poncirus trifoliata
GISH, AFLP and PCR-RFLP analysis of an intergeneric somatic hybrid combining Goutou sour orange and Poncirus trifoliata
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狗头酸橙与枳实属间体细胞杂种的GISH、AFLP和PCR-RFLP分析
DOI：
10.1007/s00299-004-0828-5
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发表时间：
2004-07
期刊：
影响因子：
--
通讯作者：
Deng XX
中科院分区：
文献类型：
--
作者：
Fu CH;Chen CL;Guo WW;Deng XX
构头酸橙子与柑桔属间体细胞杂种的研究与枳橙子枳壳Poncirus trifoliata（L.）通过电融合技术获得了Raf]，并利用扩增片段长度多态性（AFLP）、基因组原位杂交（GISH）和PCR-限制性片段长度多态性（PCR-RFLP）分析了它们的遗传特性。培养20 d后，获得16个小愈伤组织，在EME 500培养基上均发育成胚状体。在芽诱导培养基上进行几次继代培养，总培养期为6个月，芽再生。植株生长旺盛，根系发达，具有三叶草的叶片特征。倍性分析证实所有再生体均为四倍体（2 n=4 x=36），与预期相符。GISH分析证实，与大多数对称的体细胞杂种植株一样，有18条染色体来自枳橙子，其余18条来自狗头酸橙子;此外，在一株植株中还观察到染色体易位。对16个再生体及其融合亲本的AFLP分析表明，除1个体细胞杂种外，其余体细胞杂种的遗传一致。用通用引物对体细胞杂种细胞质基因组进行分析，发现其叶绿体DNA（cpDNA）带型与叶肉亲本三叶橙子相同，而线粒体（mt）基因组则与愈伤组织亲本酸橙子相同。探讨了GISH在柑橘体细胞杂种分析中的应用潜力。
Intergeneric somatic hybrids combining Goutou sour orange (Citrus aurantium L.) with trifoliate orange Poncirus trifoliata (L.) Raf] were produced by electrofusion and their genetic inheritance analyzed by amplified fragment length polymorphism (AFLP), genomic in situ hybridization (GISH), and PCR-restriction fragment length polymorphism (PCR-RFLP). Sixteen mini-calluses were obtained after 20 days of culture; they all developed into embryoids on EME500 medium. Following several subcultures on shoot induction medium for a total culture period of 6 months, shoots regenerated. The plants grew vigorously with a well-developed root system and exhibited the trifoliate leaf character of P. trifoliata. Ploidy analysis verified that all of the regenerates were tetraploids (2 n=4 x=36) as expected. GISH analysis confirmed that 18 chromosomes came from trifoliate orange and the remaining 18 from Goutou sour orange, as with most symmetric somatic hybrid plants; moreover, chromosome translocations were also observed in one plant. AFLP analysis of 16 regenerates and their fusion parents indicated that all of the somatic hybrids except one were genetically uniform. Analysis of the somatic hybrid cytoplasmic genomes with universal primers revealed that their chloroplast DNA (cpDNA) banding patterns were identical to those of the mesophyll parent trifoliate orange, while their mitochondria (mt) genomes were of the callus parent sour orange. The potential of GISH in Citrus somatic hybrid analysis is discussed.
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影响因子：
6.2
作者：
Cheng, YJ;Guo, WW;Deng, XX
通讯作者：
Deng, XX
影响因子：
6.2
作者：
M. Scarano;L. Abbate;S. Ferrante;S. Lucretti;N. Tusa
通讯作者：
M. Scarano;L. Abbate;S. Ferrante;S. Lucretti;N. Tusa
影响因子：
1.9
作者：
E. Louzada;Jude W. Grosseti;F. Gmitter;B. Nielsen;J. L. Chandler;X. Deng;N. Tusa
通讯作者：
E. Louzada;Jude W. Grosseti;F. Gmitter;B. Nielsen;J. L. Chandler;X. Deng;N. Tusa
影响因子：
6.2
作者：
K. Takami;A. Matsumara;M. Yahata;T. Imayama;H. Kunitake;H. Komatsu
通讯作者：
K. Takami;A. Matsumara;M. Yahata;T. Imayama;H. Kunitake;H. Komatsu
DOI：
--
发表时间：
1969-01
期刊：
--
影响因子：
--
作者：
T. Murashige;D. Tucker
通讯作者：
T. Murashige;D. Tucker