Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosum L.) cytoplasmic male sterility line and its maintainer line
Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosum L.) cytoplasmic male sterility line and its maintainer line
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大葱细胞质雄性不育系及其保持系中雄性不育相关 ATP 酶同工酶和 atpA 基因的比较分析
DOI：
10.1016/j.scienta.2018.08.020
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发表时间：
2019-01
影响因子：
4.3
通讯作者：
Yongqin Wang
中科院分区：
文献类型：
--
作者：
Qianchun Liu;Changlong Wen;Hong Zhao;Yongqin Wang
细胞质雄性不育（CMS）是一种母系遗传的性状，其特征是不能产生功能性花粉，对作物杂种优势的利用具有重要意义，但对大葱CMS及其育性恢复的分子机制仍知之甚少。先前通过RNA-Seq鉴定了一些CMS相关基因作为介导大葱雄性不育的候选基因。本研究测定了三种水稻幼苗的ATP酶同工酶和ATP酶活性。我们还进行了二维电泳筛选ATP酶相关蛋白。ATP合酶亚基α被发现在CMS系中特异性表达。此外，我们还发现，在不育系和保持系中，theatpA基因编码序列的全长是相同的，但在翻译的蛋白质中发现了导致氨基酸替换的单核苷酸多态性（single nucleotide polymorphisms，SNPs），我们推测theatpA基因可能参与了CMS的发生调控。
Cytoplasmic male sterility (CMS) is a maternally inherited trait characterized by the inability to produce functional pollen and is important for exploiting heterosis in crop plants, but the molecular mechanisms of CMS and fertility restoration remain poorly understood in Welsh onion. Some CMS-associated genes were previously identified by RNA-Seq as candidate genes mediating male sterility in Welsh onion. In this study, the ATPase isoenzyme and ATPase activity were investigated. We also performed 2-dimensional electrophoresis to screen for ATPase-related proteins. The ATP synthase subunit alpha was found to be specifically expressed in the CMS line. Furthermore, we found that the full length of theatpAcoding sequence was the same length in the CMS line and its maintainer line, but single nucleotide polymorphisms (SNPs) that lead to amino acid substitutions in the translated proteins were found. We hypothesize that theatpAgene is involved in regulating the development of CMS.
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DOI：
10.1016/j.plaphy.2018.01.015
发表时间：
2018-04
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
Qiaoling Yuan;Ce Song;Luyao Gao;Huihui Zhang;Cuicui Yang;Jie Sheng;Jian Ren;Dian Chen;Yong Wang
通讯作者：
Qiaoling Yuan;Ce Song;Luyao Gao;Huihui Zhang;Cuicui Yang;Jie Sheng;Jian Ren;Dian Chen;Yong Wang
通过转录组测序开发大葱 (Allium fistulosum L.) 的多态性基因 SSR 标记
影响因子：
--
作者：
Liuyi Yang;Changlong Wen;Hong Zhao;Qianchun Liu;Jingjing Yang;Lecheng Liu;王永勤
通讯作者：
王永勤
DOI：
10.11963/1002-7807.gycxcz.20170630
发表时间：
2017-07
期刊：
Cotton Science
影响因子：
--
作者：
Gong Yangcang;Z. Xuelin;Wu Jianyong;Zhang Xingping;Peng Fanjia;Z. Zhigang;He Yunxin;Mei Zhengding;Zhou Degui;Xing Chao-zhu
通讯作者：
Gong Yangcang;Z. Xuelin;Wu Jianyong;Zhang Xingping;Peng Fanjia;Z. Zhigang;He Yunxin;Mei Zhengding;Zhou Degui;Xing Chao-zhu
影响因子：
2
作者：
K. Gaikwad;A. Baldev;P. Kirti;T. Mohapatra;S. R. Bhat;V. Chopra;S. Prakash
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K. Gaikwad;A. Baldev;P. Kirti;T. Mohapatra;S. R. Bhat;V. Chopra;S. Prakash
DOI：
--
发表时间：
1985-05
期刊：
--
影响因子：
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作者：
Zhufan Xie;Xiao-qing Huang
通讯作者：
Zhufan Xie;Xiao-qing Huang