Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
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DOI：
10.1371/journal.pone.0032400
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
COSI
中科院分区：
文献类型：
--
作者：
Lefevre EA;Carr BV;Inman CF;Prentice H;Brown IH;Brookes SM;Garcon F;Hill ML;Iqbal M;Elderfield RA;Barclay WS;Gubbins S;Bailey M;Charleston B;COSI
在2009年新型H1N1流感病毒的出现和全球传播之后，选择了从A/加州/07/09 H1N1毒株生产的两种A（H1N1）pdm/09流感疫苗并用于英国的国家免疫规划：含佐剂的裂解病毒粒子疫苗和无佐剂的全病毒粒子疫苗。在本研究中，我们评估了近交系大白色猪（Babraham系）接种这些疫苗后以及接种后3个月用A（H1N1）pdm/09病毒攻毒后产生的免疫应答。两种疫苗均引起强烈的抗体反应，其中包括高水平的流感特异性IgG 1和对H1病毒的血凝抑制滴度。在体外再刺激后，用含佐剂的裂解疫苗免疫诱导了显著更高的干扰素γ产生，干扰素γ产生细胞的频率增加以及CD 4 − CD 8+（细胞毒性）和CD 4 + CD 8+（辅助）T细胞的增殖。尽管两个接种组和模拟处理组中免疫应答的幅度和广度存在显著差异，但活病毒攻毒后，在所有猪的鼻腔中均检测到相似量的病毒RNA。本研究为使用猪作为人类流感感染的有效实验模型提供了支持，包括评估治疗干预措施的保护效果。
Following the emergence and global spread of a novel H1N1 influenza virus in 2009, two A(H1N1)pdm/09 influenza vaccines produced from the A/California/07/09 H1N1 strain were selected and used for the national immunisation programme in the United Kingdom: an adjuvanted split virion vaccine and a non-adjuvanted whole virion vaccine. In this study, we assessed the immune responses generated in inbred large white pigs (Babraham line) following vaccination with these vaccines and after challenge with A(H1N1)pdm/09 virus three months post-vaccination. Both vaccines elicited strong antibody responses, which included high levels of influenza-specific IgG1 and haemagglutination inhibition titres to H1 virus. Immunisation with the adjuvanted split vaccine induced significantly higher interferon gamma production, increased frequency of interferon gamma-producing cells and proliferation of CD4−CD8+ (cytotoxic) and CD4+CD8+ (helper) T cells, after in vitro re-stimulation. Despite significant differences in the magnitude and breadth of immune responses in the two vaccinated and mock treated groups, similar quantities of viral RNA were detected from the nasal cavity in all pigs after live virus challenge. The present study provides support for the use of the pig as a valid experimental model for influenza infections in humans, including the assessment of protective efficacy of therapeutic interventions.
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影响因子：
3.7
作者：
Kistner O;Crowe BA;Wodal W;Kerschbaum A;Savidis-Dacho H;Sabarth N;Falkner FG;Mayerhofer I;Mundt W;Reiter M;Grillberger L;Tauer C;Graninger M;Sachslehner A;Schwendinger M;Brühl P;Kreil TR;Ehrlich HJ;Barrett PN
通讯作者：
Barrett PN
影响因子：
3.7
作者：
Brookes SM;Núñez A;Choudhury B;Matrosovich M;Essen SC;Clifford D;Slomka MJ;Kuntz-Simon G;Garcon F;Nash B;Hanna A;Heegaard PM;Quéguiner S;Chiapponi C;Bublot M;Garcia JM;Gardner R;Foni E;Loeffen W;Larsen L;Van Reeth K;Banks J;Irvine RM;Brown IH
通讯作者：
Brown IH
影响因子：
3.8
作者：
Lange, Elke;Kalthoff, Donata;Vahlenkamp, Thomas W.
通讯作者：
Vahlenkamp, Thomas W.
影响因子：
9.4
作者：
Spackman, E;Senne, DA;Suarez, DL
通讯作者：
Suarez, DL
影响因子：
5.5
作者：
Tachedjian, M;Boyle, JS;Andrew, ME
通讯作者：
Andrew, ME