An automated homecage system for multiwhisker detection and discrimination learning in mice.
An automated homecage system for multiwhisker detection and discrimination learning in mice.
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一种用于小鼠多维斯克检测和歧视学习的自动归宿系统。
DOI：
10.1371/journal.pone.0232916
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Barth AL
中科院分区：
文献类型：
--
作者：
Bernhard SM;Lee J;Zhu M;Hsu A;Erskine A;Hires SA;Barth AL
对啮齿动物进行自动化的家养行为训练有许多优点：压力小，与实验人员的互动很少，并且可以很容易地进行操作以适应不同的实验条件。我们已经开发了一种廉价的，基于arduino的，家庭笼训练装置，用于在自由移动的老鼠中进行感觉联想训练，使用多须气流刺激和水奖励。在1-2天的训练中，动物很容易学会这项任务，并且随着训练的进行，它们的表现会逐渐提高。我们研究了使用不同刺激强度、方向和奖励价来调节任务习得的参数。学习是通过比较预期舔刺激与无刺激（空白）试验。在高刺激强度（bbb90psi）下，动物对任务的参与明显减少。相反，非常弱的气流强度（1-2 psi）不足以产生快速学习行为。在中等刺激强度（5-6 psi）下，大多数小鼠在24-48小时的训练后了解到多须刺激预测了水奖励。暴露于异氟醚和缺少胡须都会降低动物学习任务的能力。在中等刺激强度的训练后，小鼠能够在较低的刺激强度下转移学习行为，这是感知学习的一个指标。老鼠学会了快速而准确地区分两个刺激方向，即使刺激之间的角距离小于15度。将奖励转换为更理想的奖励，阿斯巴甜，对学习轨迹几乎没有影响。我们的研究结果表明，在自动化家居环境中，触觉联想任务可以通过预期舔舐来监测，以揭示快速和渐进的行为变化。这些基于arduino的自动化小鼠笼实现了高通量训练，便于对大量转基因小鼠进行有针对性的神经活动操作分析。
Automated, homecage behavioral training for rodents has many advantages: it is low stress, requires little interaction with the experimenter, and can be easily manipulated to adapt to different experimental conditions. We have developed an inexpensive, Arduino-based, homecage training apparatus for sensory association training in freely-moving mice using multiwhisker air current stimulation coupled to a water reward. Animals learn this task readily, within 1–2 days of training, and performance progressively improves with training. We examined the parameters that regulate task acquisition using different stimulus intensities, directions, and reward valence. Learning was assessed by comparing anticipatory licking for the stimulus compared to the no-stimulus (blank) trials. At high stimulus intensities (>9 psi), animals showed markedly less participation in the task. Conversely, very weak air current intensities (1–2 psi) were not sufficient to generate rapid learning behavior. At intermediate stimulus intensities (5–6 psi), a majority of mice learned that the multiwhisker stimulus predicted the water reward after 24–48 hrs of training. Both exposure to isoflurane and lack of whiskers decreased animals’ ability to learn the task. Following training at an intermediate stimulus intensity, mice were able to transfer learning behavior when exposed to a lower stimulus intensity, an indicator of perceptual learning. Mice learned to discriminate between two directions of stimulation rapidly and accurately, even when the angular distance between the stimuli was <15 degrees. Switching the reward to a more desirable reward, aspartame, had little effect on learning trajectory. Our results show that a tactile association task in an automated homecage environment can be monitored by anticipatory licking to reveal rapid and progressive behavioral change. These Arduino-based, automated mouse cages enable high-throughput training that facilitate analysis of large numbers of genetically modified mice with targeted manipulations of neural activity.
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影响因子：
16.2
作者：
Bellavance, Marie-Andree;Takatoh, Jun;Deschenes, Martin
通讯作者：
Deschenes, Martin
影响因子：
2.5
作者：
Glazewski, S;Fox, K
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Fox, K
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作者：
Dehnhardt, G;Mauck, B;Bleckmann, H
通讯作者：
Bleckmann, H
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作者：
Clarkson JM;Dwyer DM;Flecknell PA;Leach MC;Rowe C
通讯作者：
Rowe C
影响因子：
2.5
作者：
Gess, Austen;Schneider, David M.;Vyas, Akshat;Woolley, Sarah M. N.
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Woolley, Sarah M. N.