The potential use of low-frequency tones to locate regions of outer hair cell loss.
The potential use of low-frequency tones to locate regions of outer hair cell loss.
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DOI：
10.1016/j.heares.2016.09.006
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发表时间：
2016-12
期刊：
影响因子：
2.8
通讯作者：
Chertoff ME
中科院分区：
文献类型：
--
作者：
Kamerer AM;Diaz FJ;Peppi M;Chertoff ME
目前用于诊断耳蜗性听力损失的方法在确定各种耳蜗结构的解剖损伤的位置和程度方面存在局限性。在以前的实验中，我们已经使用在圆形窗口记录的电位-耳蜗反应(CR)-来预测沙土鼠外毛细胞受损的位置。在后续实验中，我们将10 mM哇巴因应用于圆形窗龛，以减少神经活动，以量化神经对CR的贡献。我们的结论是，762赫兹音调的相当大比例的CR来自于基础听神经纤维的锁相活动，这可能会玷污我们关于外毛细胞健康的结论。然而，在如此高的浓度下，哇巴因可能也影响了外毛细胞的反应，夸大了我们认为是听神经的影响。在这项研究中，我们将哇巴因的浓度降低到1 mM，并用失真产物耳声发射来确定其对外毛细胞的生理效应。除了量化1 mM哇巴因对听神经和外毛细胞的影响外，我们还试图通过使用45和85赫兹的近次声刺激频率来减少神经对CR的贡献，并假设这些低频刺激将产生累积幅度函数(CAF)，该函数可以比762刺激更准确地反映尖端毛细胞的损伤。应用1 mM哇巴因后1小时，CR波幅显著增加，但在高通滤波噪声条件下保持不变，提示在如此低的频率下，基础听神经纤维对CR的贡献有限。
Current methods used to diagnose cochlear hearing loss are limited in their ability to determine the location and extent of anatomical damage to various cochlear structures. In previous experiments, we have used the electrical potential recorded at the round window –the cochlear response (CR) –to predict the location of damage to outer hair cells in the gerbil. In a follow-up experiment, we applied 10 mM ouabain to the round window niche to reduce neural activity in order to quantify the neural contribution to the CR. We concluded that a significant proportion of the CR to a 762 Hz tone originated from phase-locking activity of basal auditory nerve fibers, which could have contaminated our conclusions regarding outer hair cell health. However, at such high concentrations, ouabain may have also affected the responses from outer hair cells, exaggerating the effect we attributed to the auditory nerve. In this study, we lowered the concentration of ouabain to 1 mM and determined the physiologic effects on outer hair cells using distortion-product otoacoustic emissions. As well as quantifying the effects of 1 mM ouabain on the auditory nerve and outer hair cells, we attempted to reduce the neural contribution to the CR by using near-infrasonic stimulus frequencies of 45 and 85 Hz, and hypothesized that these low-frequency stimuli would generate a cumulative amplitude function (CAF) that could reflect damage to hair cells in the apex more accurately than the 762 stimuli. One hour after application of 1 mM ouabain, CR amplitudes significantly increased, but remained unchanged in the presence of high-pass filtered noise conditions, suggesting that basal auditory nerve fibers have a limited contribution to the CR at such low frequencies.
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影响因子：
2.4
作者：
LASZLO, CA;MILSUM, JH;GANNON, RP
通讯作者：
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DOI：
10.1007/s10162-014-0447-y
发表时间：
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影响因子：
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