Taurine Increases Insulin Expression in STZ-Treated Rat Islet Cells In Vitro.
Taurine Increases Insulin Expression in STZ-Treated Rat Islet Cells In Vitro.
复制标题
牛磺酸在体外增加 STZ 处理的大鼠胰岛细胞中的胰岛素表达。
DOI：
10.1007/978-94-024-1079-2_28
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
胡建民
中科院分区：
文献类型：
--
作者：
林树梅;吴高峰;赵冬冬;韩杰;杨群辉;冯颖;刘梅;杨建成;胡建民
本研究旨在探讨牛磺酸对STZ处理的体外培养胰岛细胞胰岛素的影响及其作用途径。在实验中，通过用胶原酶V原位灌注从胰腺组织中分离胰岛细胞。将在RPMI 1640培养基中维持的胰岛细胞分为6组：C：对照，E：M、T1、T2、T3组分别给予STZ处理，同时加入10 mmol/L牛磺酸（0.5mmol/L），同时T1、T2、T3组加入牛磺酸30 min，离心收集培养液，放免法检测胰岛素水平，Hanks液冲洗细胞，分别以0、10、0、5、10、C、E、M、T1、T2、T3组分别加入20 mmol/L牛磺酸24 h。提取总RNA，采用半定量RT-PCR方法扩增胰岛素基因及其转录调控因子PDX-1、NeuroD 1。结果显示，牛磺酸可抑制STZ处理的胰岛细胞胰岛素的释放，STZ组胰岛素、PDX-1和NeuroD 1基因表达显著降低，牛磺酸可上调STZ处理的胰岛细胞胰岛素、PDX-1和NeuroD 1基因表达。结论：牛磺酸对STZ处理的胰岛细胞有一定的保护和重建作用。
This research aims at figure out the effects and the pathway of taurine on insulin in islet cells cultured in vitro treated by STZ. In the experiment, islet cells were isolated from pancreatic tissue by in situ perfusion with collagenase V. The pancreatic islet cells, maintained in RPMI 1640 culture medium were divided into six groups: C: control, E: supplemented with 10 mmol/L of taurine, group M, T1, T2 and T3 was treated with STZ (0.5 mmol/L), at the same time, taurine were added in group T1,T2 and T3 for 30 min, and then culture medium were collected by centrifugation and then insulin levels were detected by radioimmunoassay, the cells were then rinsed with Hanks, and 0,10, 0, 5, 10, 20 mmol/L of taurine in group C, E, M, T1, T2 and T3 were added for 24 h respectively. Total RNA was extracted, then insulin gene and its transcription regulator such as PDX-1, NeuroD1 were amplified by semi-quantitative RT-PCR. The results showed that, the release of insulin from islet cells treated by STZ could be inhibited by taurine, gene expression of insulin, PDX-1 and NeuroD1 in STZ group decreased significantly, which were up-regulated by taurine administration. In conclusion, taurine exerts a certain degree of protective and reconstructive effects on islet cells treated by STZ.
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影响因子：
3.6
作者：
S. K. Rana;T. Sanders
通讯作者：
S. K. Rana;T. Sanders
DOI：
10.1093/ajcn/71.1.54
发表时间：
2000
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Yutaka Nakaya;A. Minami;N. Harada;S. Sakamoto;Y. Niwa;M. Ohnaka
通讯作者：
Yutaka Nakaya;A. Minami;N. Harada;S. Sakamoto;Y. Niwa;M. Ohnaka
影响因子：
2.7
作者：
A. Nandhini;V. Thirunavukkarasu;M. Ravichandran;C. Anuradha
通讯作者：
A. Nandhini;V. Thirunavukkarasu;M. Ravichandran;C. Anuradha
影响因子：
--
作者：
Hsiang-Po Huang;K. Chu;E. Némoz-Gaillard;Dorit Elberg;M. Tsai
通讯作者：
Hsiang-Po Huang;K. Chu;E. Némoz-Gaillard;Dorit Elberg;M. Tsai
影响因子：
5
作者：
H. Tokunaga;Y. Yoneda;K. Kuriyama
通讯作者：
H. Tokunaga;Y. Yoneda;K. Kuriyama