High-speed extended-volume blood flow measurement using engineered point-spread function
High-speed extended-volume blood flow measurement using engineered point-spread function
复制标题
使用工程点扩散功能进行高速大容量血流测量
DOI：
10.1364/boe.9.006444
复制
发表时间：
2018
影响因子：
3.4
通讯作者：
A. Harvey
中科院分区：
文献类型：
--
作者：
Yongzhuang Zhou;V. Zickus;Paul Zammit;Jonathan M. Taylor;A. Harvey
生物体内血流的实验表征对于理解心血管系统的发育和功能至关重要，但目前还没有报道过对大容量、高精度的厚样本进行快照成像的技术。我们结合了计算显微镜和airy光束的无衍射、自弯曲特性，在受精后3天（dpf）斑马鱼胚胎的流动血液中，通过扩展轴向范围（高达600 μm）以亚微米精度跟踪荧光珠。在通过主血管和节间血管的过程中记录了示踪珠在流动血液中的空间轨迹，并且发现轨迹与使用选择平面照明显微镜（SPIM）记录的血管分割一致。这种方法提供了足够精确的三维血流空间和时间测量，有可能直接探测关键的生物力学量，如壁面剪切应力，以及探索心血管疾病的流体影响。虽然我们展示了血液流动技术，但深度范围的十倍增强为流体流动的高速精密测量的广泛应用提供了改进，从微流体到细胞动力学测量再到宏观气溶胶表征。
Experimental characterization of blood flow in living organisms is crucial for understanding the development and function of cardiovascular systems, but there has been no technique reported for snapshot imaging of thick samples in large volumes with high precision. We have combined computational microscopy and the diffraction-free, self-bending property of Airy-beams to track fluorescent beads with sub-micron precision through an extended axial range (up to 600 μm) within the flowing blood of 3 days post-fertilization (dpf) zebrafish embryos. The spatial trajectories of the tracer beads within flowing blood were recorded during transit through both cardinal and intersegmental vessels, and the trajectories were found to be consistent with the segmentation of the vasculature recorded using selective-plane illumination microscopy (SPIM). This method provides sufficiently precise spatial and temporal measurement of 3D blood flow that has the potential for directly probing key biomechanical quantities such as wall shear stress, as well as exploring the fluidic repercussions of cardiovascular diseases. Although we demonstrate the technique for blood flow, the ten-fold better enhancement in the depth range offers improvements in a wide range of applications of high-speed precision measurement of fluid flow, from microfluidics through measurement of cell dynamics to macroscopic aerosol characterizations.
影响因子：
2
作者：
Craig, Michael P.;Gilday, Steven D.;Hove, Jay R.
通讯作者：
Hove, Jay R.
影响因子：
3.8
作者：
Iftimia, Nicusor V.;Hammer, Daniel X.;Ferrante, Anthony A.
通讯作者：
Ferrante, Anthony A.