Effect of Human Beta Defensin-2 in Epithelial Cell Lines Infected with Respiratory Viruses
Effect of Human Beta Defensin-2 in Epithelial Cell Lines Infected with Respiratory Viruses
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人 Beta Defensin-2 对呼吸道病毒感染上皮细胞系的影响
DOI：
10.4172/1948-593x.1000135
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
C. Gutiérrez
中科院分区：
文献类型：
--
作者：
M. Morales;Alej;R. Gutierrez;Dora Patricia Rosete Olvera;C. Gutiérrez
β-防御素是参与炎症过程和感染的抗菌分子家族。在人类气道中，β-防御素-2 （hβD-2）在细菌和真菌感染中表现最好；然而，它在病毒感染中的研究还不够充分。呼吸道合胞病毒（RSV）和腺病毒（ADV）是引起急性呼吸道感染的重要病原体。本研究的目的是在体外检测感染ADV和RSV的HEp-2细胞和A549细胞中hβD-2的产生和抗病毒活性；RT-PCR检测不同时间h - β d -2的产生，Western blot检测h - β d -2抗体的存在。这种防御素对病毒复制的影响是用重组hβD-2在斑块试验中确定的。结果表明，在感染ADV或RSV后，细胞系中hβ d -2的产生与感染时间成正比。使用高浓度的重组hβD-2导致病毒对细胞的有害作用较小。结果表明，无论病毒是否被包膜，这两种病毒都能诱导hβD-2的产生，并且hβD-2的存在降低了RSV和adv的复制和体外细胞病变效应。低致病性病毒或活病毒疫苗产生hβD-2可作为一些传染病的治疗工具。
β-defensins are a family of antimicrobial molecules involved in inflammatory processes and infections. In human airways, β-defensin-2 (hβD-2) is the best characterized in bacterial and fungal infections; however, it has been insufficiently studied in viral infections. The respiratory syncytial virus (RSV) and adenoviruses (ADV) are important agents of acute respiratory infections. The aim of this study was to measure in vitro the production and antiviral activity of hβD-2 in HEp-2 cells and A549 cells infected with ADV and RSV; hβD-2 production at different times was assessed by RT-PCR, and its presence by immunodetection assay (Western blot) using antibodies anti-hβD-2. The effect of this defensin on viral replication was determined using recombinant hβD-2 in plaque assays. The results revealed that in the cell lines production of hβD-2is up regulated after ADV or RSV infection, in direct proportion to the exposure time to each virus. The use of a high concentration of recombinant hβD-2 resulted in less deleterious viral effect on the cells. The results suggest that both viruses induce hβD-2 production, no matter if the virus is enveloped or not, and that presence of hβD-2 reduces replication and cytopathic in vitro effect of RSV and ADV. The hβD-2 production by low pathogenicity viruses or live viral vaccines can be useful as therapeutic tools in some infectious diseases.
DOI：
--
发表时间：
1998
影响因子：
11.1
作者：
P. K. Singh;H. Jia;K. Wiles;J. Hesselberth;L. Liu;B. Conway;E. Greenberg;E. Valore;M. Welsh
通讯作者：
P. K. Singh;H. Jia;K. Wiles;J. Hesselberth;L. Liu;B. Conway;E. Greenberg;E. Valore;M. Welsh
影响因子：
56.9
作者：
D. Yang;O. Chertov;S. N. Bykovskaia;Q. Chen;M. J. Buffo;J. Shogan;M. Anderson;J. M. Schröder-J.-M.-S
通讯作者：
D. Yang;O. Chertov;S. N. Bykovskaia;Q. Chen;M. J. Buffo;J. Shogan;M. Anderson;J. M. Schröder-J.-M.-S
人β-防御素-3类似物的膜破坏性抗菌活性。
影响因子：
6.7
作者：
Sudheendra, U. S.;Dhople, Vishnu;Datta, Aritreyee;Kar, Rajiv K.;Shelburne, Charles E.;Bhunia, Anirban;Ramamoorthy, Ayyalusamy
通讯作者：
Ramamoorthy, Ayyalusamy