An improved method for murine laser-induced choroidal neovascularization lesion quantification from optical coherence tomography images.
An improved method for murine laser-induced choroidal neovascularization lesion quantification from optical coherence tomography images.
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DOI：
10.1016/j.mex.2022.101809
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发表时间：
2022
期刊：
影响因子：
1.9
通讯作者：
Corson, Timothy W.
中科院分区：
文献类型：
--
作者：
Jensen, Nathan R.;Lambert-Cheatham, Nathan;Hartman, Gabriella D.;Muniyandi, Anbukkarasi;Park, Bomina;Sishtla, Kamakshi;Corson, Timothy W.
脉络膜新生血管的进展可以通过L-CNV和OCT上病变的定量来研究。以前的OCT定量方法是新颖和简单的，但需要许多天来评估许多病变。这种新的半自动化方法效率高，耗时少得多。激光诱导的脉络膜新生血管（L-CNV）在小鼠模型中是一种标准的方法，用于评估治疗，遗传学和致盲眼病新生血管或“湿”年龄相关性黄斑变性相关的机制。这些病变的离体评价涉及共聚焦显微镜分析。通过光学相干断层扫描（OCT）的体内评价先前已经建立，并允许病变发展的纵向评估。然而，为了产生可靠的数据，可能需要对许多病变进行评估，这可能是一个缓慢而艰巨的过程。先前的手动方法用于从正交OCT图像将这些病变量化为椭圆体是有效的，但耗时。因此，我们开发了一种简化、精简且耗时较少的OCT病变量化。
Choroidal neovascularization progression can be studied through L-CNV and quantification of lesions on OCT. A previous method of OCT quantification was novel and simple, yet required many days to evaluate many lesions. The new, semi-automated method is efficient and much less time-consuming. Laser-induced choroidal neovascularization (L-CNV) in murine models is a standard method for assessing therapies, genetics, and mechanisms relevant to the blinding eye disease neovascular or “wet” age-related macular degeneration. The ex vivo evaluation of these lesions involves confocal microscopy analysis. In vivo evaluation via optical coherence tomography (OCT) has previously been established and allows longitudinal assessment of lesion development. However, to produce robust data, evaluation of many lesions may be required, which can be a slow, arduous process. A prior, manual method for quantifying these lesions as ellipsoids from orthogonal OCT images was effective but time consuming. We therefore developed an OCT lesion quantification that is simplified, streamlined, and less time-consuming.
影响因子：
8.6
作者：
Sishtla, Kamakshi;Lambert-Cheatham, Nathan;Lee, Bit;Han, Duk Hee;Park, Jaehui;Pasha, Sheik Pran Babu Sardar;Lee, Sanha;Kwon, Sangil;Muniyandi, Anbukkarasi;Park, Bomina;Odell, Noa;Waller, Sydney;Park, Il Yeong;Lee, Soo Jae;Seo, Seung-Yong;Corson, Timothy W.
通讯作者：
Corson, Timothy W.
影响因子：
2.3
作者：
Sulaiman, Rania S.;Quigley, Judith;Corson, Timothy W.
通讯作者：
Corson, Timothy W.
影响因子：
3.7
作者：
Gong Y;Li J;Sun Y;Fu Z;Liu CH;Evans L;Tian K;Saba N;Fredrick T;Morss P;Chen J;Smith LE
通讯作者：
Smith LE