Facile synthesis of nitrogen-doped graphene for measuring the releasing process of hydrogen peroxide from living cells
Facile synthesis of nitrogen-doped graphene for measuring the releasing process of hydrogen peroxide from living cells
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轻松合成氮掺杂石墨烯用于测量活细胞过氧化氢的释放过程
DOI：
10.1039/c2jm16929k
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发表时间：
2012-03
影响因子：
--
通讯作者：
Cai, Chenxin1
中科院分区：
文献类型：
--
作者：
Wu, Ping1;Qian, Yingdan1;Du, Pan2;Zhang, Hui1;Cai, Chenxin1
调控石墨烯的电子特性对于改善和扩大其应用具有重要的技术意义。用其他元素进行化学掺杂是实现这一目标的一种有前途的方法。这项工作报告了在低温下轻松合成氮掺杂石墨烯（N-石墨烯）的方法。该方法包括石墨氧化、剥离和以肼为还原剂的化学还原步骤，可以同时实现氧化石墨烯的还原和氮原子掺杂石墨烯。光谱结果表明，可以制备N/C原子比高达~4.5%的N-石墨烯，掺杂N原子由吡啶、吡咯、石墨和氧化氮结构组成，表面原子组成分别为~28%、49%、19%和4%。所制备的N-石墨烯对H2O2还原表现出优异的电催化活性，并且基于密度泛函理论（DFT）计算详细解释了掺杂N原子对增强电催化活性的贡献。此外，N-石墨烯还进一步用于研究中性粒细胞、RAW 264.7巨噬细胞和MCF-7细胞等活细胞释放H2O2（活细胞中活性氧ROS的常见代表）的动态过程。这里提出的结果开辟了合成 N-石墨烯的新方法，并开发了一个新平台，用于可靠收集细胞 ROS 释放的动力学信息。这项工作中建立的方法可能有助于研究生理学和病理学中各种刺激的下游生物效应。
Modulating the electronic characteristics of graphene is of great technological importance for improving and expanding its applications. Chemical doping with other elements is a promising way to achieve this goal. This work reports a facile synthesis of nitrogen-doped graphene (N-graphene) at low temperature. This method, which involves the steps of graphite oxidation, exfoliation, and chemical reduction with the use of hydrazine as a reducing agent, can simultaneously realize the reduction of graphene oxide and doping graphene with nitrogen atoms. The spectroscopic results demonstrate that N-graphene with N/C atomic ratio up to ∼4.5% can be prepared, and the doping N atoms consist of pyridinic, pyrrolic, graphitic, and oxidized nitrogen structures with the surface atomic compositions of ∼28%, 49%, 19%, and 4%, respectively. The prepared N-graphene exhibits superior electrocatalytic activity toward H2O2 reduction, and the contribution of the doped N atoms to the enhanced electrocatalytic activity is explained in detail based on density functional theory (DFT) calculations. Moreover, N-graphene is further used to study the dynamic process of H2O2 (a common representative of reactive oxygen species, ROS, in living cells) release from living cells such as neutrophil, RAW 264.7 macrophage, and MCF-7 cells. The results presented here open a new way to synthesize N-graphene, and also developed a new platform for a reliable collection of kinetic information on cellular ROS release. The approach established in this work could be potentially useful in study of downstream biological effects of various stimuli in physiology and pathology.
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