Mobility of antimicrobial resistance across serovars and disease presentations in non-typhoidal Salmonella from animals and humans in Vietnam.
Mobility of antimicrobial resistance across serovars and disease presentations in non-typhoidal Salmonella from animals and humans in Vietnam.
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DOI：
10.1099/mgen.0.000798
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发表时间：
2022-05
影响因子：
3.9
通讯作者：
Mather, Alison E.
中科院分区：
文献类型：
--
作者：
Bloomfield, Samuel;Vu Thuy Duong;Ha Thanh Tuyen;Campbell, James, I;Thomson, Nicholas R.;Parkhill, Julian;Hoang Le Phuc;Tran Thi Hong Chau;Maskell, Duncan J.;Perron, Gabriel G.;Nguyen Minh Ngoc;Lu Lan Vi;Adriaenssens, Evelien M.;Baker, Stephen;Mather, Alison E.
非伤寒 沙门 (NTS)是全球细菌性小肠结肠炎的主要原因，但也导致侵入性血流感染。抗生素耐药性（AMR）阻碍了这些感染的治疗，了解AMR如何在NTS之间传播可能有助于制定有效的策略。我们调查了与来自越南的动物和人类的侵袭性疾病、肠道疾病和无症状携带相关的NTS分离株。分离株包括多种血清型以及常见和罕见的表型AMR谱;使用长和短读段测序研究与表型AMR谱相关的遗传机制和基因组背景。我们证明了大多数AMR基因型和表型之间的一致性，但确定了临床相关表型的大基因型多样性和AMR基因（ARG）在这种情况下的高迁移率潜力。我们发现84%的ARG位于质粒上，最常见的是含有IncHI1A_1和IncHI 1B（R27）_1_R27复制子的那些（33%），以及含有IncHI2_1和IncHI2A_1复制子的那些（31%）。 绝大多数（95%）的ARGS被发现在IS 6/IS 26元件的10 kbp内，这为质粒提供了在质粒和基因组的其他部分之间交换ARG的机制。在One Health背景下应用靶向长读段测序的全基因组测序确定了相对有限数量的与AMR相关的插入序列和质粒复制子。因此，在越南NTS的背景下，以及可能在其他环境中，ARG移动的机制有助于比特定ARG更成功的AMR谱，促进细菌适应不同的环境或选择压力。
Non-typhoidal Salmonella (NTS) is a major cause of bacterial enterocolitis globally but also causes invasive bloodstream infections. Antimicrobial resistance (AMR) hampers the treatment of these infections and understanding how AMR spreads between NTS may help in developing effective strategies. We investigated NTS isolates associated with invasive disease, diarrhoeal disease and asymptomatic carriage in animals and humans from Vietnam. Isolates included multiple serovars and both common and rare phenotypic AMR profiles; long- and short-read sequencing was used to investigate the genetic mechanisms and genomic backgrounds associated with phenotypic AMR profiles. We demonstrate concordance between most AMR genotypes and phenotypes but identified large genotypic diversity in clinically relevant phenotypes and the high mobility potential of AMR genes (ARGs) in this setting. We found that 84 % of ARGs identified were located on plasmids, most commonly those containing IncHI1A_1 and IncHI1B(R27)_1_R27 replicons (33%), and those containing IncHI2_1 and IncHI2A_1 replicons (31%). The vast majority (95%) of ARGS were found within 10 kbp of IS6/IS26 elements, which provide plasmids with a mechanism to exchange ARGs between plasmids and other parts of the genome. Whole genome sequencing with targeted long-read sequencing applied in a One Health context identified a comparatively limited number of insertion sequences and plasmid replicons associated with AMR. Therefore, in the context of NTS from Vietnam and likely for other settings as well, the mechanisms by which ARGs move contribute to a more successful AMR profile than the specific ARGs, facilitating the adaptation of bacteria to different environments or selection pressures.
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影响因子：
168.9
作者：
Feasey, Nicholas A.;Dougan, Gordon;Kingsley, Robert A.;Heyderman, Robert S.;Gordon, Melita A.
通讯作者：
Gordon, Melita A.
DOI：
10.1073/pnas.2003407117
发表时间：
2020-10-06
影响因子：
11.1
作者：
David S;Cohen V;Reuter S;Sheppard AE;Giani T;Parkhill J;European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group;ESCMID Study Group for Epidemiological Markers (ESGEM);Rossolini GM;Feil EJ;Grundmann H;Aanensen DM
通讯作者：
Aanensen DM
影响因子：
3.9
作者：
Harmer, Christopher J.;Hall, Ruth M.
通讯作者：
Hall, Ruth M.
影响因子：
6.4
作者：
FIERER, J;KRAUSE, M;GUINEY, D
通讯作者：
GUINEY, D
影响因子：
3.7
作者：
Matthews TD;Edwards R;Maloy S
通讯作者：
Maloy S