A new 400-kD protein from isolated adherens junctions: its localization at the undercoat of adherens junctions and at microfilament bundles such as stress fibers and circumferential bundles.
A new 400-kD protein from isolated adherens junctions: its localization at the undercoat of adherens junctions and at microfilament bundles such as stress fibers and circumferential bundles.
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DOI：
10.1083/jcb.109.6.2905
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发表时间：
1989-12
期刊：
影响因子：
--
通讯作者：
Tsukita S
中科院分区：
文献类型：
--
作者：
Tsukita S;Itoh M;Tsukita S
在先前的研究中，我们成功地从大鼠肝脏中分离出细胞间粘附连接（Tsukita， S., and S. Tsukita. 1989）。[j] .细胞生物学杂志。108:31-41。在这项研究中，我们获得了针对400-kD蛋白的单克隆抗体，该蛋白被鉴定为分离粘附连接的底涂层的主要成分之一。免疫印迹分析表明，该蛋白存在于各种类型的组织中。免疫荧光显微镜和免疫电镜显示，该蛋白不仅分布在粘附连接处的底涂层上，也分布在与非肌肉细胞连接处相关的肌动蛋白束上：培养成纤维细胞中的应力纤维和上皮细胞的周束。部分纯化的蛋白质分子看起来像一个细长的杆，长度约为400纳米。由于它的分子形状，我们将这种蛋白质命名为“tenuin”（来自拉丁语“tenuis”，薄的或细长的）。
In the previous study, we succeeded in isolating the cell-to-cell adherens junctions from rat liver (Tsukita, S., and S. Tsukita. 1989. J. Cell Biol. 108:31-41.). In this study, we have obtained mAbs specific to the 400-kD protein, which was identified as one of the major constituents of the undercoat of isolated adherens junctions. Immune blot analyses showed that this protein occurs in various types of tissues. Immunofluorescence microscopy and immune electron microscopy have revealed that this protein is distributed not only at the undercoat of adherens junctions but also along actin bundles associated with the junction in nonmuscle cells: stress fibers in cultured fibroblasts and circumferential bundles in epithelial cells. The partially purified protein molecule looks like a slender rod approximately 400 nm in length. By virtue of its molecular shape, we have named this protein 'tenuin' (from Latin 'tenuis', thin or slender).
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影响因子：
2.7
作者：
MARUYAMA, K;KIMURA, S;KUWANO, Y
通讯作者：
KUWANO, Y
细胞接触区域中的微丝组织中心：细胞附着和运动过程中的细胞骨架相互作用。
影响因子：
7.8
作者：
Geiger, B;Avnur, Z;Rinnerthaler, G;Hinssen, H;Small, V J
通讯作者：
Small, V J
DOI：
10.1083/jcb.95.1.310
发表时间：
1982-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Owaribe K;Masuda H
通讯作者：
Masuda H
DOI：
10.1073/pnas.77.7.4127
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GEIGER, B;TOKUYASU, KT;SINGER, SJ
通讯作者：
SINGER, SJ
影响因子：
2.9
作者：
DEBLAS, AL;CHERWINSKI, HM
通讯作者：
CHERWINSKI, HM