Effects of Ti, PMMA, UHMWPE, and Co-Cr wear particles on differentiation and functions of bone marrow stromal cells.
Effects of Ti, PMMA, UHMWPE, and Co-Cr wear particles on differentiation and functions of bone marrow stromal cells.
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DOI：
10.1002/jbm.a.34595
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发表时间：
2013-10
影响因子：
4.9
通讯作者：
Yang, Shang-You
中科院分区：
文献类型：
--
作者：
Jiang, Yunpeng;Jia, Tanghong;Gong, Weiming;Wooley, Paul H.;Yang, Shang-You
本研究探讨了骨科生物材料颗粒(钛、聚甲基丙烯酸甲酯、超高相对分子质量聚乙烯、钴铬)对骨髓基质细胞分化和功能的影响。从BALB/c小鼠的股骨分离细胞，在含有不同剂量微米级生物材料颗粒的完全成骨细胞诱导培养液中培养。用四甲基偶氮唑盐比色法和乳酸脱氢酶(LDH)比色法检测细胞增殖和细胞毒作用。通过碱性磷酸酶(ALP)、骨钙素、RANKL、OSX和Runx2的表达来评价成骨细胞的分化和功能。用双抗体夹心法测定培养上清液中IL-1、IL-6和肿瘤坏死因子α的含量。低剂量的钛、超高相对分子质量聚乙烯和钴铬颗粒可显著促进骨髓细胞的增殖，而高剂量的钴铬颗粒则显著抑制细胞的生长(p<；0.05)。低剂量PMMA或UHMWPE颗粒(0.63 mg/ml)对细胞有较强的碱性磷酸酶(ALP)活性，而钛、钴铬组的影响最小(p<；0.05)。UHMWPE和钛颗粒也促进了促炎细胞因子的高表达。实时荧光定量聚合酶链式反应结果显示，与高浓度(3 mg/ml)颗粒物相比，低剂量(0.5 mg/ml)颗粒物处理的细胞RANKL表达明显降低。综上所述，不同类型的磨屑颗粒对成骨细胞的分化、成熟和功能有不同的作用，颗粒颗粒相互作用的骨髓基质细胞可能在磨屑相关的无菌性假体松动的发病机制和过程中发挥重要作用。
The current study investigates the roles of orthopaedic biomaterial particles (Ti, PMMA, UHMWPE, Co-Cr) on the differentiation and functions of bone marrow stromal cells (BMSCs). Cells were isolated from femurs of BALB/c mice and cultured in complete osteoblast-induction medium in presence of micron-sized biomaterial particles at various doses. MTT assay and lactate dehydrogenase (LDH) assay were performed for cell proliferation and cytotoxicity. Differentiation and function of osteoblasts were evaluated by alkaline phosphatase (ALP), osteocalcin, RANKL, OSX, and Runx2 expressions. Murine IL-1, IL-6 and TNFα in culture media were determined by ELISA. Challenge with low doses of Ti, UHMWPE, or Co-Cr particles markedly promoted the bone marrow cell proliferation while high dose of Co-Cr significantly inhibited cell growth (p<0.05). Cells challenged with low dose of PMMA or UHMWPE particles (0.63 mg/ml) exhibited strong ALP activity, whereas Ti, and Co-Cr groups showed minimal effects (p<0.05). UHMWPE and Ti particles also promoted higher expression of pro-inflammatory cytokines. Real-time PCR data suggested that cells treated with low dose (0.5mg/ml) particles resulted in distinctly diminished RANKL expression compared to those exposed to high concentrated (3mg/ml) particles. In conclusion, various types of wear debris particles behaved differently in the differentiation, maturation, and functions of osteogenic cells; and the particulate debris-interacted bone marrow stromal cells may play an important role in the pathogenesis and process of the debris-associated aseptic prosthetic loosening.
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影响因子：
4.1
作者：
Liu, Yaling;Wang, Liping;Fatahi, Reza;Kronenberg, Mark;Kalajzic, Ivo;Rowe, David;Li, Yingcui;Maye, Peter
通讯作者：
Maye, Peter
影响因子：
14
作者：
Sethi, RK;Neavyn, MJ;Shanbhag, AS
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影响因子：
14
作者：
Granchi, D;Amato, I;Giunti, A
通讯作者：
Giunti, A
DOI：
10.1097/01.blo.0000229365.57985.96
发表时间：
2006-12-01
影响因子：
4.2
作者：
Hundric-Haspl, Zeljka;Pecina, Marko;Jukic, Irena
通讯作者：
Jukic, Irena
影响因子：
3.8
作者：
Stea, S;Visentin, M;Toni, A
通讯作者：
Toni, A