The bacterial community of the lone star tick (Amblyomma americanum).
The bacterial community of the lone star tick (Amblyomma americanum).
复制标题
孤星星蜱的细菌群落。
DOI：
10.1186/s13071-020-04550-z
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发表时间：
2021-01-14
影响因子：
3.2
通讯作者：
Zurek L
中科院分区：
文献类型：
--
作者：
Maldonado-Ruiz LP;Neupane S;Park Y;Zurek L
孤星蜱(Amblyomma Americanum)是传播多种人类和动物病原体的重要媒介，在美国东部和中西部非常常见。众所周知，扁虱携带非致病细菌，这可能在它们对病原体的媒介能力中发挥作用。之前几项使用高通量测序(HTS)技术的研究报告称，硬蜱中肠中的共生细菌丰富多样。相比之下，在我们对收集的成年孤星蜱进行的初步调查中，我们发现可培养/活细菌的数量非常低。我们的目标是通过一种平行的依赖于培养的方法和一种应用于单个扁虱的非培养的方法来分析美洲钩端螺旋体的细菌群落。我们分析了在堪萨斯州东部采集的94只成年雌性硬蜱，发现60.8%的硬蜱没有可培养的细菌，其余的硬蜱只携带26个属的67.7CFU± 42.8CFU/只。16SRRNA基因的HTS导致了总共32个操作分类单位(OTU)，其中优势内共生属Coxiella和立克次体(>； 95%)。剩余的OTU丰度很低，是典型的土壤细菌类群，表明其起源于环境。未发现CFU丰度与非培养方法的相对丰度之间存在相关性。这表明，HTS检测到的许多可培养分类群不是通过培养依赖的方法检测到的，或者是不处于可培养状态的。总体而言，我们的HTS结果表明，美洲曲霉的中肠细菌群落非常差，没有核心微生物组，大多数细菌是内共生的。
The lone star tick (Amblyomma americanum), an important vector of a wide range of human and animal pathogens, is very common throughout the East and Midwest of the USA. Ticks are known to carry non-pathogenic bacteria that may play a role in their vector competence for pathogens. Several previous studies using the high throughput sequencing (HTS) technologies reported the commensal bacteria in a tick midgut as abundant and diverse. In contrast, in our preliminary survey of the field collected adult lone star ticks, we found the number of culturable/viable bacteria very low. We aimed to analyze the bacterial community of A. americanum by a parallel culture-dependent and a culture-independent approach applied to individual ticks. We analyzed 94 adult females collected in eastern Kansas and found that 60.8% of ticks had no culturable bacteria and the remaining ticks carried only 67.7 ± 42.8 colony-forming units (CFUs)/tick representing 26 genera. HTS of the 16S rRNA gene resulted in a total of 32 operational taxonomic units (OTUs) with the dominant endosymbiotic genera Coxiella and Rickettsia (> 95%). Remaining OTUs with very low abundance were typical soil bacterial taxa indicating their environmental origin. No correlation was found between the CFU abundance and the relative abundance from the culture-independent approach. This suggests that many culturable taxa detected by HTS but not by culture-dependent method were not viable or were not in their culturable state. Overall, our HTS results show that the midgut bacterial community of A. americanum is very poor without a core microbiome and the majority of bacteria are endosymbiotic.
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影响因子：
5.7
作者：
Estrada-Peña A;Cabezas-Cruz A;Pollet T;Vayssier-Taussat M;Cosson JF
通讯作者：
Cosson JF
影响因子：
2.7
作者：
Aivelo, Tuomas;Norberg, Anna;Tschirren, Barbara
通讯作者：
Tschirren, Barbara
影响因子：
2.1
作者：
Heise, Stephanie R.;Elshahed, M. S.;Little, S. E.
通讯作者：
Little, S. E.
影响因子：
3.2
作者：
Hernandez-Jarguin, Angelica;Diaz-Sanchez, Sandra;de la Fuente, Jose
通讯作者：
de la Fuente, Jose
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro