High expression of the MADS-box gene VRT2 increases the number of rudimentary basal spikelets in wheat.
High expression of the MADS-box gene VRT2 increases the number of rudimentary basal spikelets in wheat.
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DOI：
10.1093/plphys/kiac156
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发表时间：
2022-06-27
期刊：
影响因子：
7.4
通讯作者：
Uauy, Cristobal
中科院分区：
文献类型：
--
作者：
Backhaus, Anna E.;Lister, Ashleigh;Tomkins, Melissa;Adamski, Nikolai M.;Simmonds, James;Macaulay, Iain;Morris, Richard J.;Haerty, Wilfried;Uauy, Cristobal
小穗是禾本科花序的基本组成部分，其发育和分枝方式决定了禾本科植物的花序结构和籽粒产量。在小麦（Triticum aestivum）中，中央小穗产生最多和最大的籽粒，而小穗的大小在顶部和基部逐渐减小，从而形成小麦穗的特征性披针形形状。顶向梯度与小穗的发育年龄相对应;然而，基部小穗首先发育，并且其小尺寸和不发育的原因尚不清楚。在这里，我们采用了G&T-seq，一种低输入的转录组学方法，来表征在建立披针形形状之前和之后的单个尖峰的空间部分内的基因表达谱。我们观察到一个单一的穗的顶端，中央和基底节之间的基因表达谱的差异比任何部分属于连续的发展时间点。我们发现，短营养期MADS盒转录因子，包括营养生殖转换2（VRT-A2），表达最高的麦穗基部和显示相反的表达梯度开花E类SEPALLATA 1基因。基于多年的田间试验和转基因株系，我们表明，VRT-A2在穗基部的高表达与基本基部小穗数量的增加有关。我们的研究结果，支持的计算模型，表明，延迟过渡的基础小穗从营养花发育程序的结果在披针形的小麦穗。这项研究突出了空间分辨转录组学的价值，以深入了解草花序的发育遗传学途径。大的转录梯度存在于小麦穗内，并与基本小穗发育有关，导致小麦穗的特征性披针形形状。
Spikelets are the fundamental building blocks of Poaceae inflorescences, and their development and branching patterns determine the various inflorescence architectures and grain yield of grasses. In wheat (Triticum aestivum), the central spikelets produce the most and largest grains, while spikelet size gradually decreases acropetally and basipetally, giving rise to the characteristic lanceolate shape of wheat spikes. The acropetal gradient corresponds with the developmental age of spikelets; however, the basal spikelets are developed first, and the cause of their small size and rudimentary development is unclear. Here, we adapted G&T-seq, a low-input transcriptomics approach, to characterize gene expression profiles within spatial sections of individual spikes before and after the establishment of the lanceolate shape. We observed larger differences in gene expression profiles between the apical, central, and basal sections of a single spike than between any section belonging to consecutive developmental time points. We found that SHORT VEGETATIVE PHASE MADS-box transcription factors, including VEGETATIVE TO REPRODUCTIVE TRANSITION 2 (VRT-A2), are expressed highest in the basal section of the wheat spike and display the opposite expression gradient to flowering E-class SEPALLATA1 genes. Based on multi-year field trials and transgenic lines, we show that higher expression of VRT-A2 in the basal sections of the spike is associated with increased numbers of rudimentary basal spikelets. Our results, supported by computational modeling, suggest that the delayed transition of basal spikelets from vegetative to floral developmental programs results in the lanceolate shape of wheat spikes. This study highlights the value of spatially resolved transcriptomics to gain insights into developmental genetics pathways of grass inflorescences. Large transcriptional gradients exist within a wheat spike and are associated with rudimentary basal spikelet development, resulting in the characteristic lanceolate shape of wheat spikes.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
DOI：
10.1093/plcell/koab243
发表时间：
2021-12-03
期刊：
The Plant cell
影响因子：
--
作者：
Li K;Debernardi JM;Li C;Lin H;Zhang C;Jernstedt J;Korff MV;Zhong J;Dubcovsky J
通讯作者：
Dubcovsky J
不同浓度和温度下丁香酚对不同规格草虾的麻醉效果
影响因子：
4.6
作者：
Li Y;She Q;Han Z;Sun N;Liu X;Li X
通讯作者：
Li X
影响因子：
7.4
作者：
Feng, Nan;Song, Gaoyuan;Mao, Long
通讯作者：
Mao, Long
影响因子：
5.1
作者：
Giolai, Michael;Verweij, Walter;Clark, Matthew D.
通讯作者：
Clark, Matthew D.